International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 12, Issue:2, February, 2023

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2023.12(2): 125-134

Analysis of Plasmodium falciparum Resistance to Chloroquine in Côte d'Ivoire after 20 Years: High Prevalence of Wild Strains
Kouman Kouamé Bouatini Angélo1, 2*, Ako Ako Aristide Bérenger2,Trebissou Jonhson Noël David1, Assi Serge-Brice3, 4, Oléfongo Dagnogo1,Koui Tosséa Stephane Armand1, 2, N’guessan Tiacoh Landry5, Adji Gbessi Eric1, 2,Yao Serge Stephane2, Beourou Sylvainand Toure Offianan André5
1Department of Biosciences, Felix Houphouët Boigny University, Abidjan, Côte d’Ivoire
2Malaria Unit, Institut Pasteur Côte d’Ivoire
3National Malaria Control Program, Côte d'Ivoire
4Pierre Richet Institut, Bouaké, Côte d'Ivoire
5Parasitology Unit, Institut Pasteur Côte d'Ivoire
*Corresponding author

Malaria is a major public health problem worldwide, especially in Africa, where antimalarial drug-resistant strains are spreading. In Côte d'Ivoire, Dihydro-artemisinine Piperaquine (DHA-PPQ) has been included in the policy for simple malaria management, but key mutations of the pfcrt gene are being studied to determine the mutation points that modulate Plasmodium falciparum resistance to Piperaquine. Genomic DNA from 158 patients from the five study sites with P.falciparum malaria was extracted using Chelex 5% tween. Conventional PCR amplification of the pfcrt gene was followed by Sanger sequencing of the amplificates on the Sanger sequencing platform of the CRCHU of Quebec (Canada). Comparison of different proportions was carried out using Rstudio software version 4.1.3. Analysis of individual alleles showed a prevalence of wild strain alleles over mutant alleles at all study sites. The K76T mutation was found at relatively low prevalences at the sites: 4.5% (1/22) in Bouaké; 10% (2/20) in Yamoussoukro; 18.5% (4/20) in Ayame; 20% (5/27) in Man and 34.8% (24/69) in Anonkoua-koute. Analysis of the distribution of the wild strain haplotype (CVMNK) of the pfcrt gene showed a high proportion (55-80% prevalence) compared to mutant strain haplotypes (1-20% prevalence). The high prevalence of the CVMNK haplotype across all sentinel sites supports the potential for regained sensitivity to CQ in the treatment of simple malaria. Therefore, continued molecular surveillance and in vitro analysis of pfcrt gene polymorphism mutations is highly recommended.

Keywords: Chloroquine, CVMNK Haplotype, Plasmodium falciparum, Côte d'Ivoire

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Kouman Kouamé Bouatini Angélo, Ako Ako Aristide Bérenger, Trebissou Jonhson Noël David, Assi Serge-Brice, Oléfongo Dagnogo, Koui Tosséa Stephane Armand, N’guessan Tiacoh Landry, Adji Gbessi Eric, Yao Serge Stephane, Beourou Sylvain and Toure Offianan André. 2023. Analysis of Plasmodium falciparum Resistance to Chloroquine in Côte d'Ivoire After 20 Years: High Prevalence of Wild Strains.Int.J.Curr.Microbiol.App.Sci. 12(2): 125-134. doi:
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