National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The use of microorganisms in the bioconversion of cellulose containing waste led to elaborate study of cellulases producing microbes. The current study was carried out to find out best suitable physico-chemical parameters for maximum enzyme production from bacteria isolated from banana pseudostem waste. Out of 18 morphologically distinct isolates, B 1.2 and B 1.3 were most potent and consistent over other isolates in terms of cellulase production on solid medium. Identification was performed by Biolog method and B 1.2 was identified as Enterococcus faecium and isolate B 1.3 as Aeromonas hydrophila. Isolate B 1.2 was found to secrete maximum cellulase under submerged condition at 2 % substrate concentration while B 1.3 was found at 1 % substrate concentration. Both the Isolates had shown maximum enzyme production at 7 pH, 37° C temperature and preferred peptone as nitrogen source.
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