National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Urease is a kind of amidohydrolase produced by ureolytic microorganisms. Microbial ureases have significant application in biotechnology and are of clinical relevance. The urease producing microorganisms are conventionally screened on urea containing phenol red plates. The contrast of the zone obtained due to change in pH from light orange to pink is not very sharp and distinct. Utilization of proteins by urease producing microorganisms and prolonged incubation also increases the pH of medium resulting in false positives. In the present investigation a novel, rapid, efficient and sensitive plate assay was developed for detection and screening of urease producing microorganisms. Bacteria producing ureases were screened on nutrient urea agar whereas fungi were screened on potato dextrose urea agar medium. The plates used for detection were incubated at respective temperatures and flooded with Gram’s iodine solution. Clear zones were formed around the colonies producing urease, by which the enzyme producers and non producers could be differentiated. The present method is more accurate and suitable for screening of both bacteria and fungi producing extracellular urease. Futhermore, potent isolates can be easily detected based on the diameter of hydrolyzed zone formed.
Citations |
 |
 |
 |
 |
