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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(10): 176-186
DOI: https://doi.org/10.20546/ijcmas.2022.1110.021


Studies on biotransformation of withanolides by bacteria isolated from infected plants of Withania somnifera (Dunal)
Madhusudan1*, M. Bhushanam2 and Arun Jyothi Mathias3
1Department of Biotechnology, 2Department of Zoology,
3Department of Microbiology, Maharani Cluster University, Palace Road, Bangalore, Karnataka, India
*Corresponding author
Abstract:

Endophytic microbes are commonly seen in all the plants including medicinal plants. The endophytes enhance the ability to synthesize bioactive compounds in the associated plants apart from the production of secondary metabolites. The present investigations were on analyzing these bacterial diversity associated with different medicinal plants that synthesises the secondary metabolite compounds under biotic stress in Withania somnifera. The bacterial strains were isolated and purified from the rhizosphere roots and leaves of W. somnifera. The isolated bacterial colonies were further sub-cultured and screened to species level using 16S rRNA sequencing. From the 16S rRNA sequences obtained, the strains were found to be in close match with Bacillus velezensis, Bacillus amyloliquefaciens and Pseudomonas floridensis. Further, the cell free extracts of the cultures with substrates of Withanolides and sterols were extracted and then analysed on HPLC and TLC. Of the strains obtained, B9 and B6 strains showed different bands when compared with control. Similarly, B1strain showed unique bands when compared with control. A significant rise in the production of secondary metabolites such as withaferin A, withanolide D was seen when treated with bacterial endophytes than the control. The present study could be used to trace the application of potent bacterial endophytes to nullify the pathogenicity.


Keywords: Bacterial isolates, Withania somnifera, Withaferin A, Withanolide D, HPLC, TLC, 16SrRNA
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How to cite this article:

Madhusudan, S., M. Bhushanam and Arun Jyothi Mathias. 2022. Studies on biotransformation of withanolides by bacteria isolated from infected plants of Withania somnifera (Dunal).Int.J.Curr.Microbiol.App.Sci. 11(10): 176-186. doi: https://doi.org/10.20546/ijcmas.2022.1110.021