National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Innate immunity utilizes pattern recognition receptors (PRRs) to sense conserved pathogen-associated molecular patterns (PAMPs) expressed by various pathogenic molecules to activate the initial phase of immune response. Recognition of bacterial RNA by immune sensors induces antigen-specific immunity and secretion of proinflammatory cytokines. Cardiac myocyte dysfunction is clearly identified as underlying the acute heart failure associated with bacterial infections, sepsis, as well as chronic syndrome. Cardiac myocytes express functional PRRs and sense PAMPs directly. Although the immunostimulatory potential and receptor-mediated recognition of nonself RNA are well documented, no comprehensive analysis of differential gene expression in response to naturally occurring bacterial RNA or modified RNA has been reported. Using cDNA Microarrays, we have analyzed the differential gene expression profiles of human adult cardiac myocytes stimulated with bacterial RNA. Our analysis has revealed changes in the cardiac expression profiles of 140 genes. A large proportion of upregulated genes (100) encode proteins involved in regulating the immune responses including, proinflammatory cytokines and chemokines. A significant number of genes involved in stress signalling, homeostasis, and cardiac survival were also induced. We also identified 42 genes to be suppressed. Interestingly, genes implicated in regulation of cardiac cell cycle and transcription were among these repressed genes. Collectively, these Microarray data offer for the first time an insight into human cardiac myocytes response to immunostimulatory RNA such as bacterial RNA.
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