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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(8): 42-48
DOI: https://doi.org/10.20546/ijcmas.2022.1108.005


Molecular Identification of Banana Streak Virus in Banana through PCR
Chhavi Malik, Priyanka Pal*, Dharmendra Pratap and Deepak Panwar
Department of Genetics and Plant Breeding, Chaudhary Charan Singh University,
Meerut - 250004, U.P., India
*Corresponding author
Abstract:

Banana is the most important fruit crop such as rice, wheat, and maize. Banana (Musa spp.) is cultivated in more than 130 countries in the tropics and subtropics and is a staple food crop for millions of people. Its cultivation is affected by various diseases. Among them BSD (Banana Streak disease) caused by (Banana streak Virus) is one of the most important constraints in banana production worldwide. BSV is the causal agent of this viral disease in Banana crop (Musa spp.) It is a member of the family Caulimoviridae, genus Badnavirus. Badnaviruses have bacilliform-shaped virions, =30 X 150 nm in size and a circular, noncovalently closed dsDNA genome. BSV has a monopartite genome of ~ 7.2-7.8 Kb encoding three open-reading frames (ORFs). And causes heavy economic loss in banana cultivated areas after showing chlorotic and necrotic streaks on leaves. On the basis of symptoms such as green streaks on pseudostem, stunted growth, distorted fruit in smaller branches, leaf samples were taken for isolating genomic DNA using CTAB method with some modification in Selvarajan protocol. DNA was quantified (50ug/lit DNA). PCR amplification was performed on all isolates from IARI, New Delhi, Hastinapur (UP), and Healthy sample for early detection of virus. PCR done with the help of specific primer forward (BSMysV & RNase H 5466) and reverse (BSMysV & RNase H 6196) primers, using total extracted DNA. The Agarose gel was loaded with ladder DNA (1kb), followed by sample DNA. Among DNA samples, all showed amplification by specific primer pair of BSV. During the study it has been clear that the isolates of Meerut and IARI New Delhi were infected by BSV. So, based on this analysis, it can be concluded that, detection of virus early and proper eradication of infectious plants is very important before passing of virus to another healthy plants.


Keywords: Banana Streak virus, Badnavirus, Caulimoviridae, Cetyl trimethyl ammonium bromide, PCR
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How to cite this article:

Chhavi Malik, Priyanka Pal, Dharmendra Pratap and Deepak Panwar. 2022. Molecular Identification of Banana Streak Virus in Banana through PCR.Int.J.Curr.Microbiol.App.Sci. 11(8): 42-48. doi: https://doi.org/10.20546/ijcmas.2022.1108.005