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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(7): 260-266
DOI: https://doi.org/10.20546/ijcmas.2022.1107.031


Enzymatic Synthesis of Resveratrol Oligosaccharides (Gluco-oligosaccharides) and their Enhanced Application as Antidementia Drugs that Cross the Blood-brain Barrier (BBB)
Hiroki Hamada1*, Kohji Ishihara2, Kei Shimoda3, Atsuhito Kuboki4,Yuya Kiriake5, Ryusuke Hosoda6 and Noriyuki Uchida7
1Meisterbio Co. Ltd., 29-13Ekimoto-cho, Kita-ku, Okayama 700-0024, Japan
2Department of Life Science, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho,
Kita-ku, Okayama 700-0005, Japan
3Department of Biomedical Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi,
Oita 879-5593, Japan
4Department of Biochemistry, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho,
Kita-ku, Okayama 700-0005, Japan
5Faculty of Medicine and Health Sciences, Yamaguchi University, 1-1-1 Minamikogushi, Ube-shi, Yamaguchi 755-8505, Japan
6Department of Pharmacology, School of Medicine, Sapporo Medical University,
Sapporo 060-8556, Japan
7Department of Applied Chemistry, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo, Japan
*Corresponding author
Abstract:

This study synthesized resveratrol oligosaccharides (gluco oligosaccharides) by two-step enzymatic glycosylation, using glucosyltransferase from Phytolacca americana and cyclodextrin glucanotransferase. Although resveratrol hardly crosses the blood–brain barrier (BBB) in the mouse brain, the synthesized resveratrol oligosaccharides incorporated into the mouse brain tissue crossed the BBB. Our study indicated that resveratrol modified with oligosaccharides might have gained a BBB-crossing ability. Furthermore, during the Y-maze test using senescence-accelerated mouse prone 8, our investigations revealed that the time spent in the novel Y-maze arm by the resveratrol-oligosaccharides-treated mice was longer than that spent in the novel arm by the resveratrol-treated-mice. Therefore, this study established that since resveratrol oligosaccharides could penetrate the BBB of mouse brain samples and be incorporated into the mouse’s brain tissue, they could also enhance spatial learning.


Keywords: Resveratrol oligosaccharides, Blood-brain barrier, Spatial learning
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How to cite this article:

Hiroki Hamada, Kohji Ishihara, Kei Shimoda, Atsuhito Kuboki, Yuya Kiriake and Ryusuke Hosoda. 2022. Enzymatic Synthesis of Resveratrol Oligosaccharides (Gluco-oligosaccharides) and their Enhanced Application as Antidementia Drugs that Cross the Blood-brain Barrier (BBB).Int.J.Curr.Microbiol.App.Sci. 11(7): 260-266. doi: https://doi.org/10.20546/ijcmas.2022.1107.031