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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(7): 24-33
DOI: https://doi.org/10.20546/ijcmas.2022.1107.004


Impact of Quorum Sensing Molecules at Supra-physiological Concentrations on Virulence Factors of Multiple Drug Resistant Candida Clinical Isolates
Tarek El-Said El-Banna, Fatma Ibrahim Sonbol, Sahar Nasser Al-Kharsawy* and
Lamiaa A. Al-Madboly
Department of Pharm. Microbiology, Faculty of Pharmacy, Tanta University, Tanta, Egypt
*Corresponding author
Abstract:

Candida albicans a polymorphic fungus which have the ability to develop as yeast, pseudohyphae and/or true hyphae forms. Candida isolates (n=34) were collected from different clinical specimens and identification to the species level was performed. Isolates were subjected to antifungal susceptibility testing against (nystatin, amphotericinB, Itraconazole, fluconazole, terbinafine, and caspofungin) and determination of their minimum inhibitory concentrations (MICs) were carried out according to the European Committee of Antimicrobial Susceptibility Testing (EUCAST, 2015) strategies. Virulence factors including secreted hydrolases (phospholipase and aspartyl protease) and biofilm production were evaluated. The hyphal form of Candida is themain role in infection process during its invasion to mucosal membrane. A group of genes participate in monitoring of hyphae formation in C. albicans, includingsap5 and hwp1.Farnesol compound is a quorum sensing molecule which prevents converting of yeast-to-hyphae form. The aim of our study was to determine farnesol effect in yeast-to-hyphae shift, and its related gene expressions in C. albicans using qRT-PCR. Real time-PCR analysis revealed that 1/2 MIC of farnesol has significantly decreased the expressionofsap5and hwp1genes in comparison to control group (p<0.05).


Keywords: Candida albicans, virulence, Farnesol, sap5, hwp1
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How to cite this article:

Tarek El-Said El-Banna, Fatma Ibrahim Sonbol, Sahar Nasser Al-Kharsawy and Lamiaa A. Al-Madboly. 2022. Impact of Quorum Sensing Molecules at Supra-physiological Concentrations on Virulence Factors of Multiple Drug Resistant Candida Clinical Isolates.Int.J.Curr.Microbiol.App.Sci. 11(7): 24-33. doi: https://doi.org/10.20546/ijcmas.2022.1107.004