'' '' '' '' Xpert MTB/RIF Assay: An Initial Analysis of Errors, Indeterminate and Invalid Results from Central India
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles                      Volume : 11, Issue:3, March, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(3): 285-290
DOI: https://doi.org/10.20546/ijcmas.2022.1103.032


Xpert MTB/RIF Assay: An Initial Analysis of Errors, Indeterminate and Invalid Results from Central India
Nikita Panwalkar  iD, Ankur Pauranik, Arun Kumar Sharma, Shaina Beg Mirza,Rajeev Ranjan and Prabha Desikan  iD*
Department of Microbiology, Bhopal Memorial Hospital and Research Centre,Raisen Bypass Road, Karond, Bhopal, India
*Corresponding author
Abstract:

Early diagnosis and treatment is the key to successful treatment outcome in patients with tuberculosis (TB). Xpert MTB/RIF assay has greatly reduced the lag time for diagnosis and treatment of TB patients. However, despite the high sensitivity and specificity of the Xpert MTB/RIF assay, test failure can occur due to various reasons. We aim to identify the reasons for errors, indeterminate, invalids and no results that may be useful for initiating corrective measures for Xpert MTB/RIF assay. A total of 1052 smear negative samples, including pulmonary(69.9%) and extra-pulmonary(53.3%) samples were tested using GeneXpert MTB/RIF instrument. In pulmonary samples on initial testing: 3.13% indeterminate, 2.17% no results, 2.17% invalids and 11.7% errors were seen. In extra-pulmonary samples on initial testing 2.52% indeterminates, 1.57% no results, 0.31% invalids and 12.6% errors were seen. The reasons for test failures were identified as inadequate amount or quality of reagent, failed fluid transfer, equipment malfunction, power failures, temperature related errors and errors associated with Xpert-computer communication. Invalid tests were mostly associated with quality of sputum samples. For most of the test failure events, the troubleshooting steps are simple and can be curtailed by collecting good quality sample and scrupulously following the test protocol.


Keywords: Central India, Indeterminate and Invalid Results, Test failures, Xpert MTB/RIF
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How to cite this article:

Nikita Panwalkar, Ankur Pauranik, Arun Kumar Sharma, Shaina Beg Mirza, Rajeev Ranjan and Prabha Desikan. 2022. Xpert MTB/RIF Assay: An Initial Analysis of Errors, Indeterminate and Invalid Results from Central India.Int.J.Curr.Microbiol.App.Sci. 11(3): 285-290. doi: https://doi.org/10.20546/ijcmas.2022.1103.032
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.