National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Early diagnosis and treatment is the key to successful treatment outcome in patients with tuberculosis (TB). Xpert MTB/RIF assay has greatly reduced the lag time for diagnosis and treatment of TB patients. However, despite the high sensitivity and specificity of the Xpert MTB/RIF assay, test failure can occur due to various reasons. We aim to identify the reasons for errors, indeterminate, invalids and no results that may be useful for initiating corrective measures for Xpert MTB/RIF assay. A total of 1052 smear negative samples, including pulmonary(69.9%) and extra-pulmonary(53.3%) samples were tested using GeneXpert MTB/RIF instrument. In pulmonary samples on initial testing: 3.13% indeterminate, 2.17% no results, 2.17% invalids and 11.7% errors were seen. In extra-pulmonary samples on initial testing 2.52% indeterminates, 1.57% no results, 0.31% invalids and 12.6% errors were seen. The reasons for test failures were identified as inadequate amount or quality of reagent, failed fluid transfer, equipment malfunction, power failures, temperature related errors and errors associated with Xpert-computer communication. Invalid tests were mostly associated with quality of sputum samples. For most of the test failure events, the troubleshooting steps are simple and can be curtailed by collecting good quality sample and scrupulously following the test protocol.
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