'' '' '' '' '' Evaluation of 4<sup>th</sup> Generation Elisa HIV Testing Kit and its Comparison with the 3<sup>rd</sup> Generation Kit
Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 11, Issue:3, March, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(3): 157-163
DOI: https://doi.org/10.20546/ijcmas.2022.1103.018


Evaluation of 4th Generation Elisa HIV Testing Kit and its Comparison with the 3rd Generation Kit

Swati SharmaiD*

IMA Blood Bank, Dehradun, Uttarakhand, India
*Corresponding author
Abstract:

The study was organized in the population of Dehradun, Uttarakhand at IMA Blood Bank to ascertain the sensitivity and specificity of 4th generation antibody and antigen p24 combo Elisa kit atop of 3rd generation HIV antibody Elisa kit. Methods: A total of 2045 voluntary donors were screened for HIV over a span of 6 months. The mean age group included 18 to 50 yrs with male preponderance. Each sample was assessed twain with the 3rd and the 4th generation HIV ELISA testing kit of Bio-Rad concomitantly and HIV positive samples were further authenticated with PCR. Results: Out of these 2045 samples, two samples were detected reactive with the 4th generation ELISA kit but nonreactive with the 3rd generation kit. Both these were found to be negative on PCR. One sample was found to be reactive with both 3rd and 4th generation kits and was confirmed positive by PCR. Conclusion: The study does not culminate the precedence of the 4th generation kit atop of the 3rd generation kit. However, we propound that in order to draw a logical conclusion furthermore samples should be evaluated. The study assures that the 3rd generation kit is likewise considerable as the 4th generation ELISA kit, none of the reactive samples (by 4th generation ELISA) was mislaid (by 3rd generation ELISA) which were further authenticated by PCR.


Keywords: 3rd and 4th  generation ELISA, 4th generation ELISA, HIV, AIDS, Blood donors
Download this article as Download

How to cite this article:

Swati Sharma. 2022. Evaluation of 4th Generation Elisa HIV Testing Kit and its Comparison with the 3rd Generation Kit.Int.J.Curr.Microbiol.App.Sci. 11(3): 157-163. doi: https://doi.org/10.20546/ijcmas.2022.1103.018
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.