National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study was organized in the population of Dehradun, Uttarakhand at IMA Blood Bank to ascertain the sensitivity and specificity of 4th generation antibody and antigen p24 combo Elisa kit atop of 3rd generation HIV antibody Elisa kit. Methods: A total of 2045 voluntary donors were screened for HIV over a span of 6 months. The mean age group included 18 to 50 yrs with male preponderance. Each sample was assessed twain with the 3rd and the 4th generation HIV ELISA testing kit of Bio-Rad concomitantly and HIV positive samples were further authenticated with PCR. Results: Out of these 2045 samples, two samples were detected reactive with the 4th generation ELISA kit but nonreactive with the 3rd generation kit. Both these were found to be negative on PCR. One sample was found to be reactive with both 3rd and 4th generation kits and was confirmed positive by PCR. Conclusion: The study does not culminate the precedence of the 4th generation kit atop of the 3rd generation kit. However, we propound that in order to draw a logical conclusion furthermore samples should be evaluated. The study assures that the 3rd generation kit is likewise considerable as the 4th generation ELISA kit, none of the reactive samples (by 4th generation ELISA) was mislaid (by 3rd generation ELISA) which were further authenticated by PCR.
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