National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In aquatic ecosystems there is a wide variety of viruses, including bacteriophages and others infecting plants, humans and animals. This study aimed to evaluate the viral diversity in surface water samples from three rivers located in the Amazonian mangrove, in higher and lower rainfall period. After concentration by organic flocculation, the viral nucleic acid was extracted by a commercial kit and subjected to the multiplex RT-qPCR and construction of NGS libraries. A total of 47 samples were analyzed and sixty-one different viral families were identified, with the Caudovirales order (Myoviridae, Autographiviridae, Podoviridae and Siphoviridae) being the most representative among the ten most abundant. An increase in the number of viral reads was observed during the lowest rainfall period in the analyzed rivers, with the exception of the Mocajuba river. The families composition was peculiar among the rivers evaluated during the different climatic seasons. Human enteric viruses were not found by both approaches. This study showed the great viral diversity in three different mangroves rivers and the absence of human enteric viruses, a common waterborne-viruses. More studies are needed to elucidate the virus role on the ecology of aquatic ecosystems and the biotic and abiotic impacts on viral diversity and resistance.
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