'' Viral Composition in Metagenomes of Rivers Located in the Amazon Mangrove Coast, Northeast of Pará, Brazil
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles                      Volume : 11, Issue:3, March, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(3): 1-16
DOI: https://doi.org/10.20546/ijcmas.2022.1103.001


Viral Composition in Metagenomes of Rivers Located in the Amazon Mangrove Coast, Northeast of Pará, Brazil
1Postgraduate Program in Virology, 2Virology Section, 3Center of Technological Innovation, Evandro Chagas Institute, Health Surveillance Secretariat, Brazilian Ministry of Health, BR 316 KM 07 SN, Levilândia, Ananindeua, Pará, Brazil
*Corresponding author
Abstract:

In aquatic ecosystems there is a wide variety of viruses, including bacteriophages and others infecting plants, humans and animals. This study aimed to evaluate the viral diversity in surface water samples from three rivers located in the Amazonian mangrove, in higher and lower rainfall period. After concentration by organic flocculation, the viral nucleic acid was extracted by a commercial kit and subjected to the multiplex RT-qPCR and construction of NGS libraries. A total of 47 samples were analyzed and sixty-one different viral families were identified, with the Caudovirales order (Myoviridae, Autographiviridae, Podoviridae and Siphoviridae) being the most representative among the ten most abundant. An increase in the number of viral reads was observed during the lowest rainfall period in the analyzed rivers, with the exception of the Mocajuba river. The families composition was peculiar among the rivers evaluated during the different climatic seasons. Human enteric viruses were not found by both approaches. This study showed the great viral diversity in three different mangroves rivers and the absence of human enteric viruses, a common waterborne-viruses. More studies are needed to elucidate the virus role on the ecology of aquatic ecosystems and the biotic and abiotic impacts on viral diversity and resistance.


Keywords: Viruses; virome; NGS; RT-qPCR; river; water; Brazil
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How to cite this article:

Dielle Monteiro Teixeira, Edivaldo Costa Sousa Junior, Luciana Damascena da Silva, Isis Priscila Pereira de Souza, Fernanda do Socorro Lobato Passinho, Monica Cristina de Moraes Silva, Marcio Roberto Teixeira Nunes, Jones Anderson Monteiro Siqueira, Hugo Reis Resque and Yvone Benchimol Gabbay. 2022. Viral Composition in Metagenomes of Rivers Located in the Amazon Mangrove Coast, Northeast of Pará, Brazil.Int.J.Curr.Microbiol.App.Sci. 11(3): 1-16. doi: https://doi.org/10.20546/ijcmas.2022.1103.001
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.