National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The purpose of this research was lysozyme extraction from hen egg white using SiO2. The treatment at first research was pH, at second research was the combination of mineral and ionic strength. At first research albumen from hen eggs white was diluted with buffer phosphate solution at pH 3.0; 5.0; 7.0 and 9.0 was further treated with SiO2, at second research albumen from hen eggs white was diluted with mineral (NaCl, KCl and NH4Cl) and ionic strength (0.1, 0.3 and 0.5) was further treated with SiO2. The Results showed that buffer phosphate solution at pH 3.0; 5.0; 7.0 and 9.0 produced the lysozyme extract with lysozyme activity 24578107.48, 23100847.76, 20820046.85 and 21732916.25 (U/minutes) respectively, Specific activity 1412531.5, 1271037.25, 511862.38 and 521482.71 (U/mg) respectively, Lysozyme recovery 52.20, 49.06, 44.22 and 46.16 (%) respectively, and protein content 22.32, 29.14, 40.67 and 42.15 (mg/ml) and SDS–gel electrophoresis shown several bands indicated lysozyme, ovalbumin and conalbumin. NH4Cl 0.5 M produced highest lysozyme activity (74682875.98 U/minute), specific activity (6379289.65 U/mg) and lysozyme recovery (340.86 (%)), but KCl 0.5M gave lowest lysozyme activity (30810358.01 U/minute), specific activity (2600055.60 U/mg) and lysozyme recovery (141.77 (%)), SDS–gel electrophoresis shown several bands indicated lysozyme, ovalbumin and conalbumin. Lysozyme was fairly adsorbed on the SiO2 surface at both pH 3, 5, 7 and 9, lysozyme extraction using adsoption method on SiO2 gave lysozyme with highest antibacterial activity at pH 3. Lysozyme extraction using adsoption method on SiO2 gave lysozyme with highest antibacterial activity at NH4Cl 0,05M treatment. These results strongly suggested an electrostatic nature of the adsorption behavior. Results of this study revealed that electrostatic interaction between SiO2 and lysozyme and hydrogen bonds led to binding lysozyme on SiO2.
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