International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 11, Issue:1, January, 2022

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2022.11(1): 351-360

Isolation and Characterisation of Bio-Surfactant Producing Bacteria from Oil Contaminated Soil
Pradeep Kumar Singh*, Sandesh Chibber and Veerendra Singh Nagoria
Department of Life Sciences, Rai University, Ahmedabad, Gujarat, India
*Corresponding author

Biosurfactants having the characteristics of amphipathic compounds. Biosurfactants are produced by several microorganisms which include Acinetobacter spp., Bacillus spp., Candida antarctica, Pseudomonas aeruginosa. Different screening methods, for example oil spreading assay, blood agar hemolysis, emulsification assay, foaming activity are used to select biosurfactant producing microbes. The main objective of this research work was to isolate and characterize the biosurfactant producing bacteria from oil contaminated soil. By using kerosene oil as the sole carbon source, bacterial strain was isolated and screened having the characteristics of biosurfactant properties. Number of techniques like oil spreading technique, blood hemolysis test, foaming activity, and emulsification activity were performed for the screening of the biosurfactant producing bacteria, and found positive oil spreading technique in bacterial strain B1 and B2. Strain B1 (52%) has high emulsifying activity, whereas in B2 it was around 42 %. Strain B1 showed complete breakdown of the hemoglobin of the red blood cells in the vicinity of bacterial colony i.e. β blood hemolysis test and in B2 it is lack of hemolysis i.e. γ hemolysis. It was observed that foaming stability in B2 strain is lesser than B2. Both the isolated bacterial strains B1 and B2 showed Gram positive, and they are in rod and circular shape respectively. From the result of various biochemical characterization and cell morphological characterization, the isolated strain was bacillus type bacteria.

Keywords: Biosurfactants, Blood agar, Hemolysis, Emulsification, Lipopeptides

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How to cite this article:

Pradeep Kumar Singh, Sandesh Chibber and Veerendra Singh Nagoria. 2022. Isolation and Characterisation of Bio-Surfactant Producing Bacteria from Oil Contaminated Soil.Int.J.Curr.Microbiol.App.Sci. 11(1): 351-360. doi:
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