This present study for the first time reports a facile and ecofriendly route for synthesis of Silver nanoparticles by using the extract of berries of Pimenta dioica. Extract of berries was used for reduction of silver nitrate into silver nanoparticles (AgNPs). Silver nanoparticles have unique phytochemical properties and activities compared to their whole parent material. Due to multiple applications of Silver nanoparticles (AgNPs) various biological methods are gaining recognition for their production. The silver nanoparticles were irregular spherical in shape. In the reaction mixture brown color appeared which confirmed the primary indication of Ag NPs formation and it was confirmed by using UV-visible spectroscopy which shows maximum peak intensity at 425 nm. Sample was characterized by using UV-viz Spectrophotometer, SEM, and TEM. The synthesized silver nanoparticles were spherical in clusters. Crystalline size calculated was found to be 40 nm; and TEM shows that synthesized nanoparticles having size of 94±29 nm. The Ag NPs were evaluated for their antimicrobial activity on Gram negative bacteria Escherichia coli and Gram positive Staphylococcus aureus bacteria by qualitative methods and it was confirmed.

Download this article as