Cucumber mosaic virus is omnipresent infectious pathogenic source to various vegetables including cucurbitaceous crops. In the present study the virus is isolate from infected from infected pumpkin plant for transmission. Survey was done from Distt. Dehradun during December 2018 to May 2019 to identify the occurrence of CMV on pumpkin. Leaf samples were collected from the field area and subjected to lab to investigate the presence of CMV by process of inoculating sap from infected plant of pumpkin to healthy vigorously raised seedlings of pumpkin planted in pots for experiment. The infected plant show symptoms like hypertrophy, stunting, mosaic pattern on leaves, small leaf size and chlorosis. Biophysical properties of CMV in C. pepo were determined by performing experiment. Thermal inactivation point (TIP)was 70º in mild isolate and 75º in severe isolate, Dilution end point (DEP) was 1:100,000 (10ˉ⁵) in mild isolate and 1:5,00,000 in severe isolate, Longevity ‘in vitro’ (LIV) of 5 days with 40% infection in mild isolate and 6 days with 20% infection in severe isolate was found. Longevity in vivo of9 days with 20% infection in mild isolate and 14 days with 20% infection in severe isolate was found.

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