National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is a emerged human disease during last month of 2019 in china. The ongoing Coronavirus disease-2019, many diagnostic test available (i.e., TrueNat, Rapid, CBNAAT, etc), amongst them Real Time Polymerase Chain Reaction (RT-PCR) a gold standard and most use technique for the diagnosis of COVID-19. Aim: To evaluate the four various commercial COVID-19 RT-PCR diagnostic kits. The prospective observational study conducted in the Department of Microbiology, Autonomous State Medical College, Firozabad, India, from march to August 2021. A total of 85 sample included in this study. Data should be analysis and maintain in Microsoft Excel version 2007. All 85 respiratory sample tested to four RT-PCR kits. Out of 85, 9 (10.6%) and 73 (85.9%) were negative with all kits. 3 were inconclusive with all four kits. A sensitivity of all four kits at least 100% and a specificity of 99%. Conclusion: The assay exhibited that all four kits high specificity and sensitivity for SARS-CoV-2 and good analytical performance using genes and/or virus.
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