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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(11): 120-132
DOI: https://doi.org/10.20546/ijcmas.2021.1011.016


Phenotypic and Molecular Identification of Pathogenic Fusarium Species Isolated from Various Pulse Growing Geographic Areas of India
Monika Singh1*, O. P. Sharma2 and Someshwar Bhagat3
1Mewar University, NH-79, Gangarar, Chittorgarh, Rajasthan-312901, India
2ICAR-National Research Centre for Integrated Pest Management, Lal Bahadur Shastri Building, Ajantha Ave, Pusa Campus, New Delhi - 110012, India
3ICAR-Central Rainfed Upland Rice Research Station (NRRI), Ranchi Patna Highway, Hazaribag, Jharkhand - 825302, India
*Corresponding author
Abstract:

Fusarium phythopathogenic fungi is responsible for high economic loss of cereal food crop. The objective of this study was aimed at isolation, morphological and molecular identification of Fusarium species. 13 different Fusarium spp. i.e. F. solani, F. chlamydosporum, F. tabacinum, F. fujikuroi, F. oxysporum, F. verticillioides, F. brachygibbosum, Fusarium sp. and F. incarnatum were isolated and identified from diseased samples of chickpea, pigeonpea, rice, lentil and garden pea crop. Colony characteristics like colony color, colony growth diameters, mycelium type, sporulation, pigmentation, odour were obtained after culture purification. Shape, size and septation of microconidia and macroconidia, position, shape, occurrence and size of chlamydospores, conidiophore branching were examined microscopically. MS10, BI01 and KA14 isolates were slow growing, BI02 and UP07 were moderate growing and BI03, HA04, MS06, MS09, MS11 and KA(Gul)13 were fast to very fast growing on PDA after 7-10 days. Chlamydospores were found in most of the isolates. Colonies were abundant, loosely tufted, fluffy, pannose, vinaceous floccose, powdery and some were flat appressed, arachnoid. Pigmentation of most of the isolates was pinkish white to dark pink, carmine to violet in colour. Phylogenetic analysis was done by maximum likelihood method using the ITS-rDNA region of Fusarium isolates and multiple sequence alignment of ITS DNA sequences was done using Clustal_W program and all identified sequences were submitted in NCBI GenBank database.


Keywords: Fusarium sp., identification, ITS-rDNA, molecular, morphology, phylogeny
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How to cite this article:

Monika Singh, O. P. Sharma and Someshwar Bhagat. 2021. Phenotypic and Molecular Identification of Pathogenic Fusarium Species Isolated from Various Pulse Growing Geographic Areas of India.Int.J.Curr.Microbiol.App.Sci. 10(11): 120-132. doi: https://doi.org/10.20546/ijcmas.2021.1011.016