Detection and Testing Pathogenicity of Xanthomonas axonopodis pv. punicae Causing Bacterial Blight on Pomegranate
Mahesh S. Dashyal¹*, M. P. Basavarajappa¹, G. Manjunath2, D. P. Prakash3,Sayeed Wajeed R. Mulla4 and Anita Rajkumar Ghandhe5
¹Department of Plant Pathology, College of Horticulture, University of Horticultural Sciences Bagalkot, 587104, Karnataka, India
²Department of Plant Pathology, College of Horticulture, University of Horticultural Sciences Campus, Mysuru-571130, Karnataka, India
³Department of Fruit science, College of Horticulture, University of Horticultural Sciences Campus, Koppal, Karnataka, India
4Department of Biotechnology and Crop Improvement HREC, Vijayapur, University of Horticultural Sciences, Bagalkot 587104, Karnataka, India
5Department of Soil Science and Agriculture Chemistry, University of Horticultural Sciences, Bagalkot 587104, Karnataka, India
*Corresponding author
Abstract:
Pomegranate, one of the most important fruit crops, is constantly challenged by Bacterial blight caused by Xanthomonas axonopodispv. punicae, is a prevalent and destructive pomegranate disease. Accurate diagnosis of disease is very important to manage the disease. PCR have been widely used to detect or verify the presence of pathogen in recent decades. These molecular-based methods are rapid, accurate and sensitive for detecting pathogens. In this study, a primer set KKM5 and KKM6 was used and amplification of 491bp of gyrB gene proved the presence of Xap. The pathogenicity of the Xap was confirmed following Koch’s postulates.
Keywords: Pomegranate, bacterial blight, pathogenisity, polymerase chain reaction
Download this article as
How to cite this article:
Mahesh S. Dashyal, M. P. Basavarajappa, G. Manjunath, D. P. Prakash, Sayeed Wajeed R. Mulla and Anita Rajkumar Ghandhe. 2021. Detection and Testing Pathogenicity of Xanthomonas axonopodis pv. punicae Causing Bacterial Blight on Pomegranate.
Int.J.Curr.Microbiol.App.Sci. 10(10): 43-46. doi:
https://doi.org/10.20546/ijcmas.2021.1010.006
Citations