Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(9): 560-566
DOI: https://doi.org/10.20546/ijcmas.2021.1009.064


Molecular Detection and Antimicrobial Susceptibility of Salmonella Species in Chickens
Gloria Sugun1, Ehizibolo David2, Ebenezer Bukola Ogundejii2, Kaikabo Ahmed2,Agada O. Godwin2, Benshak Audu2 and Sugun Manasa2*
1No. 7 Vaccine Complex National Veterinary Research Institute Staff Quarters Vom
2National Veterinary Research Institute Vom Plateau State Nigeria
*Corresponding author
Abstract:

The study was intended for molecular detection of Salmonella Spp isolated from chicken. A total of 160 samples comprising of 40 liver, 40 spleen, 40 lungs and 40 intestines were collected directly into buffered peptone in universal bottles at the poultry slaughter houses of the four districts in Jos South Local Government area of Plateau State. The samples were enriched in 10 ml of Rappaport-Vassiliades broth and cultured onto Xylems Lysine Deoxycholate (XLD) agar for the isolation of bacteria. The isolated bacteria were identified by studying staining characteristics, cultural properties on different selective media, biochemical tests, catalase and coagulase test, and finally by PCR. Out of 160 samples, 65 (41%) samples were found positive for Salmonella Spp on XLD and 24 (37%) positive by biochemical analysis. Two(2) Salmonella isolates were amplified by 942 bp gene based PCR. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the Salmonella isolates were resistant to amoxycillin (100%) and erythromycin (100%), gentamicin (100%), Clindamycin (100%) streptomycin (100%), tetracycline (100%), sulphamethoxazole/trimethoprim (100%) but sensitive to Ceftiofur (100%).


Keywords: Molecular detection, Antimicrobial Susceptibility, Salmonella in Chicken
Download this article as Download

How to cite this article:

Gloria Sugun, Ehizibolo David, Ebenezer Bukola Ogundejii, Kaikabo Ahmed, Agada O. Godwin, Benshak Audu and Sugun Manasa. 2021. Molecular Detection and Antimicrobial Susceptibility of Salmonella Species in Chickens.Int.J.Curr.Microbiol.App.Sci. 10(9): 560-566. doi: https://doi.org/10.20546/ijcmas.2021.1009.064