National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Antibiotic resistance is the most worrisome problem in treating bacterial infections. It is very important to maintain the clinical efficacy of vancomycin because of their critical role in preventing and treating life-threatening healthcare associated infections (HAIs). Therefore, this study aimed to determine the existence of different levels of vancomycin resistance among Staphylococcus aureus (S. aureus) strains in Surgical Departments of Tanta University Hospitals. This study was carried out on 200 patients who had evidence of infections. All specimens were cultured on blood and mannitol salt agarsand their antimicrobial susceptibility were investigated by modified Kirby-Bauer method and VITEK 2 system. Vancomycin susceptibility was assessed by E-test and vancomycin screen agar. mecA, vanA and vanB genes were investigated by polymerase chain reaction (PCR). Methicillin resistance was detected in 70% of S. aureus isolates and mecAgene was detected in 68% of these isolates. The total percentage of S. aureus with reduced vancomycin susceptibility (SA-RVS) was 20%. All isolates were negative for vanA and vanB genes. VITEK 2 system failed to detect any isolates of SA-RVS.
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