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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(8): 402-413
DOI: https://doi.org/10.20546/ijcmas.2021.1008.049


Detection of Different Levels of Resistance to Vancomycin among Staphylococcus aureus Isolated from Surgical Departments of Tanta University Hospitals
Maha Salah Elnady1*, Marwa Mohamed Ezzat1, Azza Mahmoud Hassan1,Amal Abd El Tawab Hasheesh2 and Mina Samy Messiha1
1Department of Medical Microbiology and Immunology,
2Department of Surgery, Faculty of Medicine, Tanta University, Egypt
*Corresponding author
Abstract:

Antibiotic resistance is the most worrisome problem in treating bacterial infections. It is very important to maintain the clinical efficacy of vancomycin because of their critical role in preventing and treating life-threatening healthcare associated infections (HAIs). Therefore, this study aimed to determine the existence of different levels of vancomycin resistance among Staphylococcus aureus (S. aureus) strains in Surgical Departments of Tanta University Hospitals. This study was carried out on 200 patients who had evidence of infections. All specimens were cultured on blood and mannitol salt agarsand their antimicrobial susceptibility were investigated by modified Kirby-Bauer method and VITEK 2 system. Vancomycin susceptibility was assessed by E-test and vancomycin screen agar. mecA, vanA and vanB genes were investigated by polymerase chain reaction (PCR). Methicillin resistance was detected in 70% of S. aureus isolates and mecAgene was detected in 68% of these isolates. The total percentage of S. aureus with reduced vancomycin susceptibility (SA-RVS) was 20%. All isolates were negative for vanA and vanB genes. VITEK 2 system failed to detect any isolates of SA-RVS.


Keywords: S. aureus, MRSA, vancomycin resistance, E-test, VITEK 2, SA-RVS
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How to cite this article:

Maha Salah Elnady, Marwa Mohamed Ezzat, Azza Mahmoud Hassan, Amal Abd El Tawab Hasheesh and Mina Samy Messiha. 2021. Detection of Different Levels of Resistance to Vancomycin Among Staphylococcus aureus Isolated from Surgical Departments of Tanta University Hospitals.Int.J.Curr.Microbiol.App.Sci. 10(8): 402-413. doi: https://doi.org/10.20546/ijcmas.2021.1008.049