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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(8): 164-176
DOI: https://doi.org/10.20546/ijcmas.2021.1008.021


Isolation, Identification and Characterization of Antimicrobial Activity Exhibiting Actinomycete Streptomyces paradoxus Strain KUASN-7 from Soil
R. Muthuraj, Meghashyama P. Bhat, S. B. Dhanyakumara, H. M. Halaswamy, K. N. Shashiraj, Bidhayak Chakraborty, S. S. Pallavi and Sreenivasa Nayaka*
Department of Botany, Karnatak University, Dharwad-580003, Karnataka, India
*Corresponding author
Abstract:

Soil acts as a rich source of Actinomycetes, which are generally characterized as potent antibiotic producers. The research rationale of this study was to isolate and characterize potential Actinomycetes possessing antibiotic properties from the soils of Dharwad Karnataka, India. Further, isolates were characterized using microscopic, biochemical and molecular techniques. Initially, ten different Actinomycetes were isolated on starch casein agar media. Out of ten isolates, five of them were Gram-positive and were further screened for antimicrobial potency by cross streak method. Among 5 Actinomycetes isolates studied, isolate SN-7 was found to be most efficient against all the four pathogens tested, which confirmed its antimicrobial efficacy. The potent active isolate SN-7 was identified as Streptomyces strain by 16S rRNA gene sequence similarity analysis and given a strain no. SN-7. Genebee and NEB Cutter version 2.0 online tools were used to confirm 16S rRNA gene secondary structure and restriction sites on the DNA sequence respectively, which was deposited with NCBI with an accession number KX284898, Streptomyces SN-7. In addition, Streptomyces SN-7 was subjected for preliminary screening of bioactive metabolites using column chromatography and the eluted fractions were further characterized using UV and FTIR spectroscopy techniques.


Keywords: Actinomycetes, FTIR, Antibiotics, Antimicrobial, Streptomyces
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How to cite this article:

Muthuraj, R., Meghashyama P. Bhat, S. B. Dhanyakumara, H. M. Halaswamy, K. N. Shashiraj, Bidhayak Chakraborty, S. S. Pallavi and Sreenivasa Nayaka. 2021. Isolation, Identification and Characterization of Antimicrobial Activity Exhibiting Actinomycete Streptomyces paradoxus Strain KUASN-7 from Soil.Int.J.Curr.Microbiol.App.Sci. 10(8): 164-176. doi: https://doi.org/10.20546/ijcmas.2021.1008.021