National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Soil acts as a rich source of Actinomycetes, which are generally characterized as potent antibiotic producers. The research rationale of this study was to isolate and characterize potential Actinomycetes possessing antibiotic properties from the soils of Dharwad Karnataka, India. Further, isolates were characterized using microscopic, biochemical and molecular techniques. Initially, ten different Actinomycetes were isolated on starch casein agar media. Out of ten isolates, five of them were Gram-positive and were further screened for antimicrobial potency by cross streak method. Among 5 Actinomycetes isolates studied, isolate SN-7 was found to be most efficient against all the four pathogens tested, which confirmed its antimicrobial efficacy. The potent active isolate SN-7 was identified as Streptomyces strain by 16S rRNA gene sequence similarity analysis and given a strain no. SN-7. Genebee and NEB Cutter version 2.0 online tools were used to confirm 16S rRNA gene secondary structure and restriction sites on the DNA sequence respectively, which was deposited with NCBI with an accession number KX284898, Streptomyces SN-7. In addition, Streptomyces SN-7 was subjected for preliminary screening of bioactive metabolites using column chromatography and the eluted fractions were further characterized using UV and FTIR spectroscopy techniques.
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