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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(8): 9-18
DOI: https://doi.org/10.20546/ijcmas.2021.1008.002


Comparison of Multiplex Polymerase Chain Reaction and Culture for Diagnosis of Acute Bacterial Meningitis
Sanjana Upadhyay*, Anil Kumar Bilolikar and Sukrutha Gopal Reddy
Department of Laboratory Medicine, Krishna Institute of Medical Sciences, Minister Road, Secunderabad - 500003, Telangana, India
*Corresponding author
Abstract:

Bacterial meningitis remains a serious global health problem. The laboratory plays a crucial role in diagnosing this devastating disease. It is a medical emergency that requires early rapid diagnosis to establish the specific cause for effective antimicrobial therapy. To detect and compare the etiological agents in suspected cases of bacterial meningitis by conventional culture and multiplex PCR. CSF examination was done macroscopically and microscopically (Gram stain). Biochemical analysis and cell counts were also performed followed by conventional culture using BacT/ALERT 3D Microbial Detection System and VITEK 2 COMPACT for identification and antimicrobial susceptibility testing and multiplex PCR. Bacterial growth was detected in 45 CSF samples by culture and multiplex PCR together. With bacterial culture as gold standard method, the sensitivity, specificity, positive predictive value and negative predictive value of multiplex PCR were 85.7%, 76.7%, 37.5% and 97.05% respectively. Multiplex PCR is a rapid and sensitive test for the diagnosis of bacterial meningitis. The use of molecular techniques (PCR) can provide a valuable addition in cases with high rates of culture-negative results i.e, those with administration of antibiotics prior to lumbar puncture.


Keywords: CSF, multiplex PCR, culture, Vitek 2 compact
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How to cite this article:

Sanjana Upadhyay, Anil Kumar Bilolikar and Sukrutha Gopal Reddy. 2021. Comparison of Multiplex Polymerase Chain Reaction and Culture for Diagnosis of Acute Bacterial Meningitis.Int.J.Curr.Microbiol.App.Sci. 10(8): 9-18. doi: https://doi.org/10.20546/ijcmas.2021.1008.002