National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacterial meningitis remains a serious global health problem. The laboratory plays a crucial role in diagnosing this devastating disease. It is a medical emergency that requires early rapid diagnosis to establish the specific cause for effective antimicrobial therapy. To detect and compare the etiological agents in suspected cases of bacterial meningitis by conventional culture and multiplex PCR. CSF examination was done macroscopically and microscopically (Gram stain). Biochemical analysis and cell counts were also performed followed by conventional culture using BacT/ALERT 3D Microbial Detection System and VITEK 2 COMPACT for identification and antimicrobial susceptibility testing and multiplex PCR. Bacterial growth was detected in 45 CSF samples by culture and multiplex PCR together. With bacterial culture as gold standard method, the sensitivity, specificity, positive predictive value and negative predictive value of multiplex PCR were 85.7%, 76.7%, 37.5% and 97.05% respectively. Multiplex PCR is a rapid and sensitive test for the diagnosis of bacterial meningitis. The use of molecular techniques (PCR) can provide a valuable addition in cases with high rates of culture-negative results i.e, those with administration of antibiotics prior to lumbar puncture.
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