International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:7, July, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(7): 752-764

RNA Isolation from Cell Free – Saliva of Water Buffalo
Gangu Naidu Surla1*, Lal Krishna2 and Onteru Suneel kumar2
1Department of Veterinary Biochemistry, College of Veterinary Science, Garividi-535101 Sri Venkateswara Veterinary University, Tirupati, India 2Department of Animal Biochemistry, ICAR- NDRI, Karnal – 132001, India
*Corresponding author

Non-invasive methods are easy, simple, and effortless ways of collecting resources for the detection of biomarkers in water buffaloes. Among non-invasive fluid resources, saliva is a rich source of biomarkers, unlike milk and urine samples with no time restriction to obtain a sample. Saliva is a real-time indicator of the pathophysiology of animal health status, it has a source of many biomarkers including RNA and miRNAs, Studying RNA biology is less complex as compared to other “omics” study, but the isolation of salivary extracellular RNA is difficult in water buffaloes saliva due to high alkaline pH (pH ≥ 9), feed material and microbial contamination in the oral cavity. In addition, available kits prepared for humans were also not suitable in animal studies. To address these issues, 32 different protocols which already existed were tested. Finally, a protocol has been developed first time in buffalo for isolating total RNA from cell-free saliva has A260/280 is 1.89 ± 0.24 and concentrations 25.69 ± 7.49 ng/µL. The present study, however, unfortunately, could not isolate a very high-quality RNA to meet the general canonical principles of RNA quality and quantity for NGS transcriptomics and miRnomics.

Keywords: Water buffalo – cell-free saliva – Total RNA isolation - NGS quality

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How to cite this article:

Gangu Naidu Surla, Lal Krishna and Onteru Suneel kumar. 2021. RNA Isolation from Cell Free – Saliva of Water Buffalo.Int.J.Curr.Microbiol.App.Sci. 10(7): 752-764. doi:
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.