National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Studying DNA methylation effects in Alu repetitive elements in the human genome might vary with the underlying disease state. The purpose of this study was to examine the association between Alu methylation status and complicated urinary tract infection in children. The study was conducted in the Department of Pediatrics of a referral hospital. Genomic DNA from 50 children with UTI and 50 healthy controls were isolated. The level of DNA methylation in Alu repeat was examined in these children using methylation‑specific polymerase chain reaction. Highly significant difference in the percentage of Alu DNA methylation level was found between cases and controls. The mean and standard deviation of the Alu DNA methylation level between cases (26.70±18.80) and controls (38.20±18.28) (P<0.003). The results of this study show that Alu repeats vary significantly among cases and controls. This study indicates that interactions between Alu DNA methylation and host characteristics may determine disease progression.
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