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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(7): 629-637
DOI: https://doi.org/10.20546/ijcmas.2021.1007.068


DNA Methylation of Alu Repeats in Complicated Urinary Tract Infection in Children
Kalaivani Sekar1, Sriram Krishnamurthy1*, Jharna Mandal2 and Medha Rajappa3
1Department of Pediatrics, 2Department of Microbiology,
3Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry, India
*Corresponding author
Abstract:

Studying DNA methylation effects in Alu repetitive elements in the human genome might vary with the underlying disease state. The purpose of this study was to examine the association between Alu methylation status and complicated urinary tract infection in children. The study was conducted in the Department of Pediatrics of a referral hospital. Genomic DNA from 50 children with UTI and 50 healthy controls were isolated. The level of DNA methylation in Alu repeat was examined in these children using methylation‑specific polymerase chain reaction. Highly significant difference in the percentage of Alu DNA methylation level was found between cases and controls. The mean and standard deviation of the Alu DNA methylation level between cases (26.70±18.80) and controls (38.20±18.28) (P<0.003). The results of this study show that Alu repeats vary significantly among cases and controls. This study indicates that interactions between Alu DNA methylation and host characteristics may determine disease progression.


Keywords: Alu repeats; Complicated urinary tract infection; DNA methylation; Epigenome; Methylation-specific polymerase chain reaction
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How to cite this article:

Kalaivani Sekar, Sriram Krishnamurthy, Jharna Mandal and Medha Rajappa. 2021. DNA Methylation of Alu Repeats in Complicated Urinary Tract Infection in ChildrenInt.J.Curr.Microbiol.App.Sci. 10(7): 629-637. doi: https://doi.org/10.20546/ijcmas.2021.1007.068