National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A phytase (myo-inositol-hexakisphosphate phosphohydrolase) enzyme was purified and identified from wheat varieties during process. The four wheat varieties, selected on the basis of their higher phytase activity for investigation were DBW-17, HD-2894, HUW-234 and LOK-1. Among the four selected wheat varieties, LOK -1 variety reported the maximum phytase activity in study. The three steps i.e. ammonium sulphate precipitation, dialysis and ion exchange chromatography on DEAE-cellulose, Silica gel, Ceralite IR 120, Ceralite IR 400, Dowex 1X8 and Dowex 50X8 column were adopted for phytase partial purification process. Finally, phytase enzyme was purified about 55.18 fold with 17.39% recovery by Dowex 50X8 column from LOK-1 wheat variety in ion exchange chromatography step. Molecular weight of partially purified phytase enzyme of four wheat varieties was found to be monomeric protein as approximately 65kDa mass in SDS-PAGE electrophoresis (denatured phytase) as well as in Native-PAGE electrophoresis (non-denatured phytase) when compared to ladder markers lane. This can have exhibit maximal phytate degrading activity. This enzyme can have good efficiency for industrial applications.
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