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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(7): 378-385
DOI: https://doi.org/10.20546/ijcmas.2021.1007.041


Purification of Phytase Enzyme from Four Selected Indian Wheat Varieties (Triticum aestivum L.)
Manish Kumar1, 2 and Sushma2
1Department of Biochemistry, Institute of Life Science, Bundelkhand University, Jhansi-284128, India
2Department of Biochemistry and Biochemical Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj-211007, India
*Corresponding author
Abstract:

A phytase (myo-inositol-hexakisphosphate phosphohydrolase) enzyme was purified and identified from wheat varieties during process. The four wheat varieties, selected on the basis of their higher phytase activity for investigation were DBW-17, HD-2894, HUW-234 and LOK-1. Among the four selected wheat varieties, LOK -1 variety reported the maximum phytase activity in study. The three steps i.e. ammonium sulphate precipitation, dialysis and ion exchange chromatography on DEAE-cellulose, Silica gel, Ceralite IR 120, Ceralite IR 400, Dowex 1X8 and Dowex 50X8 column were adopted for phytase partial purification process. Finally, phytase enzyme was purified about 55.18 fold with 17.39% recovery by Dowex 50X8 column from LOK-1 wheat variety in ion exchange chromatography step. Molecular weight of partially purified phytase enzyme of four wheat varieties was found to be monomeric protein as approximately 65kDa mass in SDS-PAGE electrophoresis (denatured phytase) as well as in Native-PAGE electrophoresis (non-denatured phytase) when compared to ladder markers lane. This can have exhibit maximal phytate degrading activity. This enzyme can have good efficiency for industrial applications.


Keywords: Phytase, ammonium sulphate precipitation, dialysis, ion exchange chromatography
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How to cite this article:

Manish Kumar and Sushma. 2021. Purification of Phytase Enzyme from Four Selected Indian Wheat Varieties (Triticum aestivum L.).Int.J.Curr.Microbiol.App.Sci. 10(7): 378-385. doi: https://doi.org/10.20546/ijcmas.2021.1007.041