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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 3589-3593
DOI: https://doi.org/10.20546/ijcmas.2020.905.426


Molecular Detection of Anaplasma ovis in Apparently Healthy Sheep and Goats in Tamil Nadu
M. Aravind*, S. Gowrishankar, C. Soundararajan and Bhaskaran Ravi Latha
Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India
*Corresponding author
Abstract:

Anaplasma species are obligate intracellular rickettsial pathogens transmitted by ticks with an impact on animal health. Anaplasma ovis infects sheep and goats and it can be diagnosed by different methods like Giemsa staining, PCR or competitive ELISA. In this study, a PCR method based on major surface protein 4 (MSP4) gene, was utilized for the detection of Anaplasma infection. A total of 780 field blood samples were collected of which 360 from sheep and 420 from goat. Out of 780 whole blood samples collected from sheep and goats, 81.02% (632/780) were found positive for Anaplasma ovis of which 82.50% (297/360) of sheep and 79.76% (335/420) of goats were positive. This indicates high prevalence and thus, A. ovis should be considered as an important constraint of livestock production, and further efforts are needed to better understand the epidemiology and to implement suitable control measures.


Keywords: Anaplasmaovis, Sheep and Goats, MSP4 gene and PCR
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How to cite this article:

Aravind, M., S. Gowrishankar, C. Soundararajan and Bhaskaran Ravi Latha. 2020. Molecular Detection of Anaplasma ovis in Apparently Healthy Sheep and Goats in Tamil Nadu.Int.J.Curr.Microbiol.App.Sci. 9(5): 3589-3593. doi: https://doi.org/10.20546/ijcmas.2020.905.426