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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(7): 182-199
DOI: https://doi.org/10.20546/ijcmas.2021.1007.021


A Study on Cloning and Expression of Stress Induced DREB Transcription Factor Gene from Rice (Oryza sativa)
Keshamma1*, A. C. Manjula2, P. N. Dakshayini3, Rajeev R. Kolgi1,B. Roy4 and R. Geethanjali5
1Department of Biochemistry, 2Department of Sericulture,
3Department of Zoology, Maharani Cluster University, Palace Road,
Bengaluru, Karnataka, India
1Department of Biochemistry, 4Department of Zoology and Genetics, Government Science College, Bengaluru, Karnataka, India
5Department of Botany, Maharani Cluster University, Palace Road,
Bengaluru, Karnataka, India
*Corresponding author
Abstract:

The transcription factors DREBs/CBFs specifically interact with the dehydration-responsive element/C-repeat (DRE/CRT) cis-acting element (core motif: G/ACCGAC) and control the expression of many stress inducible genes in Arabidopsis. In rice, we isolated five cDNAs for DREB homologs: OsDREB1A, OsDREB1B, OsDREB1C, OsDREB1D, and OsDREB2A. Expression of OsDREB1A and OsDREB1B was induced by cold, whereas expression of OsDREB2A was induced by dehydration and high-salt stresses. The OsDREB1A and OsDREB2A proteins specifically bound to DRE and activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts. Over-expression of OsDREB1A in transgenic Arabidopsis induced overexpression of target stress-inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought, high-salt, and freezing stresses. This indicated that OsDREB1A has functional similarity to DREB1A. However, in microarray and RNA blot analyses, some stress-inducible target genes of the DREB1A proteins that have only ACCGAC as DRE were not over-expressed in the OsDREB1A transgenic Arabidopsis. The OsDREB1A protein bound to GCCGAC more preferentially than to ACCGAC whereas the DREB1A proteins bound to both GCCGAC and ACCGAC efficiently. The structures of DREB1-type ERF/AP2 domains in monocots are closely related to each other as compared with that in the dicots. OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high-salt, and/or cold stresses.


Keywords: Oryza sativa, Abiotic, Stress, OsDREB, Arabidopsis
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How to cite this article:

Keshamma, E., A. C. Manjula, P. N. Dakshayini, Rajeev R. Kolgi, U. B. Roy and Geethanjali, R. 2021. A Study on Cloning and Expression of Stress Induced DREB Transcription Factor Gene from Rice (Oryza sativa).Int.J.Curr.Microbiol.App.Sci. 10(7): 182-199. doi: https://doi.org/10.20546/ijcmas.2021.1007.021