National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Smear microscopy still remains the bedrock of tuberculosis (TB) diagnosis, in spite of innumerable advances, particularly in developing countries. So far, ZN microscopy is the most expended amongst all methods currently employed in laboratories for diagnosis on account of its simplicity, speed, low cost, and minimal requirement of equipment1 and technical skills despite the fact that LED microscopy is a better diagnostic tool. WHO, also recommends that that LED microscopy be phased in as an alternative for conventional ZN light microscopy.2 So the present study was planned with the aim of evaluating the efficacy of LED Microscopy and a new inspiring technique “Decomics®” kit, developed and tested by Salubris Inc USA, based on absorbent beads, which does not require centrifugation and is easy to use and swift. This is the first study in India utilizing Decomics® method. A cross sectional study was conducted on 500 sputum samples from clinically suspected cases of pulmonary tuberculosis. Among the 500 clinically suspected cases of pulmonary tuberculosis, Mycobacterium tuberculosis was detected in 100 cases (20%) by both direct microscopes. Where LED microscopy gave 100% result and direct ZN light microscopy obtained 90% positivity. Efficiency of “Decomics®” as decontamination in detection of mycobacteria was better than classical and popular NaOH-NALC. Elimination of need for centrifugation decreased processing time by half and higher detection rate of mycobacteria were important advantages of Decomics®.
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