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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Shoot apex and nodal segment explants of guggul were placed on Murashige and Skoog Medium (MS medium, 1962) supplemented with different concentration of activated charcoal (20, 40, 60, 80 and 100 mg/l) and Polyvinylpyrrolidone (2, 4, 6, 8 and 10 mg/l) for shoot bud induction. Activated charcoal (20 - 100 mg/l) was added in the basal medium with responsive level of plant growth regulators (3.0 mg/l BAP) in nodal segment, maximum number of shoot bud (1.55) was observe at 60 mg/l activated charcoal with no browning intensity in the culture medium. Maximum shoot bud proliferation in nodal segment explants followed by 6 mg/l level of polyvinylpyrrolidone. Shoot apex explant was gave minimum response in activated charcoal and polyvinylpyrrolidone.
Guggul, Callus induction, Tissue culture, in vitro
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