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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 1071-1083
DOI: https://doi.org/10.20546/ijcmas.2021.1003.135


Isolation, Screening, Optimization of Cultural Conditions of Submerged Fermentation and Partial Purification of Alkaline Chitinase Enzyme from Fusarium sp.
Keshamma1*, R. Haleshappa1, U. B. Roy2, K. N. Shivakumara3 and B. T. Sridhar4
1Department of Biochemistry, Maharani Cluster University, Palace Road,Bengaluru, Karnataka, India
2Department of Biochemistry, Government Science College, Bengaluru, Karnataka, India
3Department of Zoology and Genetics, Government Science College,Bengaluru, Karnataka, India
4Department of Chemistry, Maharani Cluster University, Palace Road,Bengaluru, Karnataka, India
*Corresponding author
Abstract:

The importance of microbial chitinase has gained much interest because on the one hand, it reduces environmental hazards and on the other hand it increases production of industrially important value-added products. Hence the present study was conducted with the objective to isolate, screen and characterize chitinase producing fungi from soil samples. In the present study ten fungal isolates have been evaluated for the production of extracellular chitinase and selected one potent isolate based on maximum chitin hydrolysis and identified as Fusarium sp. SS06. Fusarium sp. KS06 has been used for the production of chitinase under submerged fermentation and partially purified and characterized. Fusarium sp. showed maximum enzyme production of at 40°C; pH 8.0 with 1.0 ml of inoculum size. Chitinase enzyme produced from Fusarium sp. was partially purified by 80% ammonium sulphate saturated and molecular weight was determined as ~38 kDa. The partially purified enzyme’s maximum activity at 25°C and pH 9.0. The partially purified chitinase was able to hydrolyse 100% of its natural substrate colloidal chitin. Whereas with regards to starch and casein only chitin hydrolysed only 30% and 21% respectively. In conclusion the extracellular chitinase producing fungi Fusarium sp. was isolated from soil sample and cultural conditions for the production of chitinase under submerged fermentation were optimized. The partially purified alkaline chitinase from Fusarium sp. can be a used as potential biocontrol agent.


Keywords: Fusarium sp. Chitinase, Purification, Biocontrol agent, Submerged fermentation
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How to cite this article:

Keshamma, E., R. Haleshappa, U. B. Roy, K. N. Shivakumara and Sridhar, B. T. 2021. Isolation, Screening, Optimization of Cultural Conditions of Submerged Fermentation and Partial Purification of Alkaline Chitinase Enzyme from Fusarium Sp.Int.J.Curr.Microbiol.App.Sci. 10(3): 1071-1083. doi: https://doi.org/10.20546/ijcmas.2021.1003.135