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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(3): 118-128
DOI: https://doi.org/10.20546/ijcmas.2021.1003.018


Isolation and Partial Characterization of Bacteriophage against Salmonella gallinarum
Shumaila Malik, Renu Chauhan, Lahari Laddika, Salauddin Qureshi*,Bablu Kumar and A. K. Tiwari
Division of Biological Standardization, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, India
*Corresponding author
Abstract:

A lytic bacteriophage (SGP-1) against Salmonella gallinarum (S. gallinarum) was isolated by an enrichment protocol from the sewage water of a poultry farm. The presence of bacteriophage was observed by spot test over the bacterial lawn and double agar overlay assay. Isolated phage was able to produce clear, circular plaques of 2-3mm diameter on S. gallinarum lawn culture. This Bacteriophage also had a lytic effect on Salmonella typhimurium. The bacteriophage SGP-1 was found to be stable at a temperature range between 4oC to 60oC for 30 min and able to survive in a wide range of pH between 2 to 11. The characterization of the bacteriophage would help establish a basis for adopting the application of the most effective bacteriophage therapeutics to control bacterial infection of poultry. 


Keywords: Salmonella Gallinarum, Bacteriophage, Double agar overlay, Host range
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How to cite this article:

Shumaila Malik, Renu Chauhan, Lahari Laddika, Salauddin Qureshi, Bablu Kumar and Tiwari, A. K. 2021. Isolation and Partial Characterization of Bacteriophage against Salmonella gallinarum.Int.J.Curr.Microbiol.App.Sci. 10(3): 118-128. doi: https://doi.org/10.20546/ijcmas.2021.1003.018