National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In present examination, Rathi cows were chosen to identified leptin gene qualities for polymorphism. Complete 160 lactating Rathi cows from livestock research station (LRS), Nohar and Bikaner were chosen. Blood samples were collected and DNA isolated by the spin column method. For exon-2 and exon 3 of LEP gene primers were constructed based on available sequences of LEP gene in the NCBI GenBank database. Fragments of 326 and 454 bp of Exon- 2 and exon 3 regions of LEP gene, respectively, were amplified by a polymerase chain reaction in a final reaction volume of 25μl. The PCR-SSCP was utilized for amplification and detection of polymorphism in LEP gene. In exon-2 of LEP gene shows monomorphism. In exon 3 of the LEP gene, two SSCP patterns were observed and it shows the polymorphic nature of this region. In exon 3 of the LEP gene two alleles and two genotypes with frequency 0.915, 0.085, 0.832 and 0.168, respectively, were observed. In present examination, exon 2 of LEP gene noticed monomorphic nature and exon 3 of LEP quality uncovered polymorphic nature in studied population of Rathi cattle.
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