National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present investigation was undertaken to standardize the embryo culture technique for plant regeneration from immature embryos in wheat-rye crosses. Embryo rescue was tried to obtain the hybrid plants. At 17-19 days after pollination, the frequency of embryo development showed that wheat cvs. Hope and Highbury were incompatible with both rye cvs. (Russian rye and Rye 8461), while UP 2338 and WH 896 were incompatible with Russian rye only. The compatibility was the highest in the cross of Chinese Spring (CS) with Russian rye. The embryos were cultured on two media combinations, viz., Taira and Larter’s modified Norstog’s (TL1 medium) and TL1 medium supplemented with 0.5 mg/l of naphthalene acetic acid (NAA) and 1.0 mg/l Kinetin (6-furfuryl amino purine) (TL2 medium) following Taira and Larter (1978). Maximum embryo survival (85.0 %) was observed in CS and CS/Hope 5B crosses with Russian rye, when cultured on Taira and Larter’s modified Norstog’s medium. Hence, it was found that Taira and Larter’s modified Norstog’s medium was the best for plant regeneration studies in wheat-rye crosses.
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