International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:1, January, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(1): 3429-3436

Isolation and Biochemical Characterization of Indigenous Rhizobium Strains from Root Nodules of Field Grown Soybean Crop
Shanti*, Diptimayee Dash and Tapas Chowdhury
Department of Agricultural Microbiology, Indira Gandhi Krishi Vishwavidyalaya,Raipur, (C.G.), India
*Corresponding author

Total of eighteen (18) nodule samples of different 18 soybean cultivars were collected. Eighteen soybean varieties were grown in the Research field of Department of Plant Breeding and Genetics, College of Agriculture, Indira Gandhi KrishiVishwavidyalaya, Raipur (C.G.) during kharif2019-20. The present study was aimed to isolate the beneficial nitrogen fixing Rhizobium from root nodules of Soybean varieties to evaluate the effective symbiosis. The isolation, morphological and biochemical characterization were carried out in the laboratory of Department of Agricultural Microbiology, College of Agriculture, Raipur. Isolated Rhizobium strains were subjected to cultural and colony morphology tests on Yeast Extract Mannitol Agar (YEMA) from which top ten isolates showing good colony growth were selected for the biochemical characterization. After series of biochemical and Sugar Fermentation tests SRh-1, SRh-03, SRh-18, SRh-137 and SRh-136were identified as promising strains. This study confirms the presence of promising indigenous Rhizobia in some Soybean varieties i.e., NRCSL 1, RSC 11-03, RKS 18, NRC 137 and NRC 136 over other varieties for effective symbiosis with indigenous rhizobia.

Keywords: Root nodules, Rhizobium, Soybean and Biochemical characterization

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Shanti, Diptimayee Dash and Tapas Chowdhury. 2021. Isolation and Biochemical Characterization of Indigenous Rhizobium Strains from Root Nodules of Field Grown Soybean Crop.Int.J.Curr.Microbiol.App.Sci. 10(1): 3429-3436. doi:
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