National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Total of eighteen (18) nodule samples of different 18 soybean cultivars were collected. Eighteen soybean varieties were grown in the Research field of Department of Plant Breeding and Genetics, College of Agriculture, Indira Gandhi KrishiVishwavidyalaya, Raipur (C.G.) during kharif2019-20. The present study was aimed to isolate the beneficial nitrogen fixing Rhizobium from root nodules of Soybean varieties to evaluate the effective symbiosis. The isolation, morphological and biochemical characterization were carried out in the laboratory of Department of Agricultural Microbiology, College of Agriculture, Raipur. Isolated Rhizobium strains were subjected to cultural and colony morphology tests on Yeast Extract Mannitol Agar (YEMA) from which top ten isolates showing good colony growth were selected for the biochemical characterization. After series of biochemical and Sugar Fermentation tests SRh-1, SRh-03, SRh-18, SRh-137 and SRh-136were identified as promising strains. This study confirms the presence of promising indigenous Rhizobia in some Soybean varieties i.e., NRCSL 1, RSC 11-03, RKS 18, NRC 137 and NRC 136 over other varieties for effective symbiosis with indigenous rhizobia.
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