National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine that plays a major role in regulating immune responses, acute phase reactions and haematopoiesis. IL-6 is produced by different cell types and acts on B cells, T cells, hepatocytes, haematopoietic progenitor cells and cells of the central nervous system. In this study the partial complementary DNA sequence (CDS) fragment of 219 bp of IL-6 gene of guinea fowl (Numidam eleagris) was PCR amplified, cloned and sequenced. This was the first report of sequencing and characterization of the IL-6 gene of guinea fowl. The nucleotide sequence (NCBI GenBank Accession No HQ258968) of IL-6 was compared for sequence homology with related sequences from different poultry species, retrieved from Genbank database and the per cent genetic similarity and genetic divergence were estimated using Megalign programme of DNASTAR software. Guinea fowl showed higher per cent identity (96.6%) with chicken in comparison to per cent identity (90.8%) with that of duck. The per cent identity between chicken and duck was comparatively less (88.5%). Likewise the IL-6 gene of duck showed low per cent divergence (9.2%) with guinea fowl in comparison to chicken IL-6 gene (11.5%). The phylogenetic analysis revealed that chicken and guinea fowl were clustered together and separated from the duck. These results suggested that guinea fowl showed high genetic similarity with chicken in comparison to duck. However, duck is evolutionary closer to guinea fowl then chicken.
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