National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A792 bp fragment of iNOS gene in guinea fowl has been successfully amplified using the primers designed from available sequence of iNOS gene of chicken. This fragment is equivalent to the chicken iNOS in size and no insertion or deletion was observed. Sizable polymorphism was observed between guinea fowl and other poultry species for iNOS nucleotide sequence. The ratio between non-synonymous to synonymous nucleotide substitutions was 1:4.6 between guinea fowl and chicken, whereas it was 1:4.3 between guinea fowl and quail. The iNOS amino acid sequence comprised of 263 amino acids in guinea fowl. The nucleotide sequence alignment of Gf-iNOS gene with the iNOS genes from other poultry species identified 7 SNPs i.e. T/A, C/T, T/C, A/T, G/A, G/A and C/T substitution at 320, 370, 409, 460, 484, 520 and 553 nt positions, respectively in our Gf-iNOS sequence. For Gf-iNOS PCR-RFLP, Mbo I, Sau3A I, Nde II and Dpn I were identified. Similarly, for PCR-RFLP of chicken iNOS, Rsa I, Eco47 III and Hae II; and for quail iNOS, Mn lI, Hpa II, Nci I, Msp I and Nsi I were identified.Based on nucleotide sequence comparisons for iNOS gene, guinea fowl showed very high and almost equal per cent identity (95.6 – 96.2) with chicken and quail. Quail and chicken also showed very high per cent identity (96.7-97.0) between them. phylogenetic analysis also revealed the similar trend. The polymorphism identified in iNOS CDS in present study, may be utilized for exploring the mechanism of higher disease resistance in guinea fowl, which may further be exploited to develop disease resistant chicken population.
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