International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:1, January, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(1): 2879-2886

Molecular Characterization and Expression Profile of Partial TLR2 Gene in Malnad Gidda Cattle
M. Satheesha1*, R. Nagaraja2, H. M. Yathish1, A. Sahadev1, R. Jayashree3,M. Kotresh3 and S. Naveenkumar1
1Dept. of Animal Genetics and Breeding, Veterinary College, Bangalore
2Veterinary College, Gadag
3Veterinary College, Shivamogga, Karnataka, India
*Corresponding author

Toll like receptors (TLRs) are the cell surface receptors which mainly act by activating innate and adaptive immunity. These TLR’s play very important role in disease resistance through the recognition of pathogen-associated molecular patterns (PAMPs). Among all the TLRs identified TLR2 is mainly involved in the recognition of multiple pathogenic microorganisms. In the present study partial region exon-2 of TLR2 gene covering 997bp region was characterized in Malnad gidda cattle which is one of the draft breed of cattle which is famous for its disease resistance property. The partial exon-2 of TLR2 gene is amplified using self designed primers and subjected for RFLP analysis using BsaA1 restriction enzyme which revealed AB genotypes with fragments of sizes 71, 926 and 997bp exhibiting monomorphic pattern. The allelic frequency of A and B allele are 0.5 and the genotypic frequency of the AB genotyope is 1. Further, the observed heterozygositie, polymorphic information content (PIC) and allelic diversity are 1.0, 0.3750 and 0.5 respectively. Sequence analysis of Malnad gidda cattle revealed three SNPs when compared to Bos Taurus sequence.

Keywords: Polymorphism, Malnad gidda, RFLP, Sequencing

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How to cite this article:

Satheesha, G. M., R. Nagaraja, H. M. Yathish, A. Sahadev, R. Jayashree, A. M. Kotresh and Naveenkumar, S. 2021. Molecular Characterization and Expression Profile of Partial TLR2 Gene in Malnad Gidda Cattle.Int.J.Curr.Microbiol.App.Sci. 10(1): 2879-2886. doi:
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