Field experiment was conducted at Department of Agronomy, College of Agriculture, Dhule during Kharif 2019 to study the effect of pre and post emergence herbicides on soil microflora in sweet corn. The per cent decrease in the bacterial, fungal and actinomycetes count was in the range of 8.26 to 9.22 % in the weed free treatment (two hand weeding), 18.57 to 24.94 % in the treatment of weedy check (T₁) and 22.48 to 30.10 % in the treatments of pre and post emergence herbicides (T₃ to T₁₀) over the initial values of 24.5 x 10⁷ cfu g^-1 soil, 8.6 x 10⁴ cfu g^-1 soil and 19 x 10⁶ cfu g^-1 soil, respectively. Application of tembotrione @ 120 g ha^-1 as post emergence herbicide (T₉) showed less adverse effect on soil bacterial population at harvest than the other herbicides treatments. Application of 2,4 D ethyl ester @ 1000 g ha^-1 as post emergence herbicide (T₁₀) showed less adverse effect on soil fungal population than the other herbicides treatments. Application of halosulfuron-methyl @ 90 g ha^-1 as post emergence herbicide (T₈) showed less adverse effect on soil actinomycetes population than the other herbicides treatments under study.

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