National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Banana bunchy top disease (BBTD) caused by Banana bunchy top virus (BBTV) is one of the most devastating diseases in banana. The virus is reported to be evolving and causing serious economic loss in various banana growing parts of the world. The complete coding region and partial untranslated region (UTR) of coat protein (CP) gene was amplified from total DNA isolated from infected samples. The partial sequence of DNA-S of seventeen Kerala isolates were analysed for molecular diversity, codon usage bias and phylogeny were deduced. A large negative value of Fu and Li’ D and F tests indicated a demographic expansion of the population. Being a DNA virus, BBTV showed very less diversity among the isolates in the coding and non-coding regions. Although, nucleotide diversity and mutation rate in the non-coding region was surprisingly higher than the coding region. In the non-coding region, the stem loop region was highly conserved responsible for binding to the master rep protein and facilitating rolling circle replication of the genome. Nevertheless, the phylogeographic analysis confirmed an association with the geography as the northern, southern and clusters were grouped separately with high bootstrap support. There was a very conspicuous codon usage bias (CUB) in the CP gene and evidently nineteen synonymous codons were favoured over the others. The role of mutation and selection pressure on CUB of the gene was established using neutrality plot and Nc plot.
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