International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:1, January, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(1): 1530-1539

SNPs within Exon VIII of DGAT1 Gene in Gaolao Cattle
N. Pantawane1, D. S. Kale1*, P. G. Koringa2 and D. V. Patil1
1Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Nagpur, India
2Department of Animal Biotechnology, College of Veterinary Science and A.H., Anand Agricultural University, Anand, India
*Corresponding author

DGAT1 gene is major candidate gene and K232A polymorphism revealed pronounced influence on fat percentage in cattle milk. It was planned to reveal polymorphisms within regions of DGAT1 gene in Gaolao cattle using PCR-RFLP and DNA Sequencing and to find association between polymorphisms and milk production traits. DNA was extracted from purebred Gaolao cattle. DGAT1G1-CfrI locus in Exon-VIII region exhibited monomorphic pattern; however DGAT1G2-BglI locus was polymorphic with frequency for the K alleles as 0.9875 and for allele A as 0.0125 in Gaolao population. The animals exhibiting PCR-RFLP as AA (K) & GC (A) genotype were sequenced which revealed one SNP T-G at 231st position. DNA sequencing of HRM analysed DGAT1G3-212 bp amplicons revealed 03 polymorphic sites as computational SNPs at 52nd position as SNP G-A, at 51st position as SNP G -A and at 67th position as SNP A-G. DGAT1G4 amplified sequence revealed SNP, C-A at 23rd position. In various studies, the allele (K) encoding the lysine variant has proved to be associated with regard to the milk fat synthesis, and which can be a useful marker for milk fat traits in selection and breeding.

Keywords: DGAT1, Gaolao Cattle, HRM, PCR-RFLP, Sequencing, SNP

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How to cite this article:

Pantawane, S. N., D. S. Kale, P. G. Koringa and Patil, D. V. 2021. SNPs within Exon VIII of DGAT1 Gene in Gaolao Cattle.Int.J.Curr.Microbiol.App.Sci. 10(1): 1530-1539. doi:
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