National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
DGAT1 gene is major candidate gene and K232A polymorphism revealed pronounced influence on fat percentage in cattle milk. It was planned to reveal polymorphisms within regions of DGAT1 gene in Gaolao cattle using PCR-RFLP and DNA Sequencing and to find association between polymorphisms and milk production traits. DNA was extracted from purebred Gaolao cattle. DGAT1G1-CfrI locus in Exon-VIII region exhibited monomorphic pattern; however DGAT1G2-BglI locus was polymorphic with frequency for the K alleles as 0.9875 and for allele A as 0.0125 in Gaolao population. The animals exhibiting PCR-RFLP as AA (K) & GC (A) genotype were sequenced which revealed one SNP T-G at 231st position. DNA sequencing of HRM analysed DGAT1G3-212 bp amplicons revealed 03 polymorphic sites as computational SNPs at 52nd position as SNP G-A, at 51st position as SNP G -A and at 67th position as SNP A-G. DGAT1G4 amplified sequence revealed SNP, C-A at 23rd position. In various studies, the allele (K) encoding the lysine variant has proved to be associated with regard to the milk fat synthesis, and which can be a useful marker for milk fat traits in selection and breeding.
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