International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 10, Issue:1, January, 2021

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2021.10(1): 632-640

Effect of Seed Priming on Specific Activity of Acid Phosphatase and -Amylase in Germinating Seeds and Correlation Study in Onion (Allium cepa L.)
B. D. Patil*, A. R. Aher, V. R. Awari and M. R. Manjare
RSJRS, Kolhapur, Mahatma Phule Krishi Vidyapeeth, Rahuri, India
*Corresponding author

An experiment was laid out in a factorial RBD with three replications involving two lots i.e. fresh and one year old onion seeds and eight seed priming treatments. The seeds were primed with zinc (0.5 and 0.75%), magnesium (0.5 and 0.75%), calcium (0.5 and 0.75%) and GA3 (50 and 100ppm) for 6 hours before laboratory germination and untreated control. The enzyme activity for acid phosphatase and a-amylase at 6hr priming of seed, 3rd day to germination, 6th day to germination, 9th day to germination and 12th day to germination was carried out in laboratory. All the treatments in fresh seed lot showed higher acid phosphatase activity upto 3rd day of germination than old seed lot. However, old seed lot treatments surpassed fresh seed lot treatments on 6th day analysis. Positive and non significant correlation up to 6 days of germination was observed in old seed lot for germination. In the case of fresh seed lot positive and non significant correlation was observed for germination. In the present investigation a-amylase activity in old untreated seed was more (0.90) as compared to fresh untreated seed (0.34). After 6 h seed priming a-amylase activity was more in old seed lot treatments than fresh seed lot treatments. In the case of old seed lot treatments a-amylase activity increased upto 6th day except in old untreated seed wherein it increased upto 9th day. In the case of old seed lot positive significant correlation for a - amylase up to 6 days of germination was found. In fresh seed lot significant correlation for a -amylase was found except at 9 days to germination.

Keywords: Onion, Allium cepa L., Seed priming, Acid Phosphatase, α-Amylase, Correlation Germination

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Onion, Allium cepa L., Seed priming, Acid Phosphatase, α-Amylase, Correlation GerminationInt.J.Curr.Microbiol.App.Sci. 10(1): 632-640. doi:
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