|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Escherichia coli are important commensal with pathogenic potentiality in bovine neonates and may cause intestinal and extra-intestinal infections. Different enteric pathogens such as E. coli may remain associated with bovine diarrhoea which is a multifactorial menace. Possession of antimicrobial resistance genes by these commensal or pathogens associated with diarrhea makes the issue more serious. The study was aimed to detect the occurrence and characterize extended-spectrum (ESBL) and AmpC (ACBL) beta-lactamases producing pathogenic E. coli (STEC) in diarrhoeal faecal samples collected from bovines. All the E. coli strains (13/21, 61.9%) isolated from bovine diarrhoea cases (n=21) were found to be ESBL producers. In total, 11 (84.6%) isolates were positive in cefoxitin–cloxacillin double-disc synergy (CC-DDS) test for AmpC production and the isolates were also confirmed by PCR for blaAmpC. Any of the E. coli isolates were not carrying the class I integrons but 7 (53.8%) of those isolates were found to possess theSTEC gene, eaeA (responsible for causing diarrhoea) as revealed in PCR assays. All the ESBL producing E. coli isolates showed a high level of resistance to amoxicillin/clavulanic acid, ceftriaxone, ceftazidime, ampicillin/cloxacillin, cefotaxime, cefepime, cefoperazone (100%), cefoxitin (84.6%) and tetracycline (61.5%) whereas Ertapenem (92.3%), and enrofloxacin (84.6%) were intermediately sensitive against these pathogens. All the isolates were susceptible to amikacin, levofloxacin, gentamicin, ampicillin/sulbactam, and doxycycline.