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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 3716-3722
DOI: https://doi.org/10.20546/ijcmas.2020.909.458


Standardization of SYBR Green Based Real Time PCR for Detection of Canine Parvovirus-2 (CPV-2)
Srinivas Manchikatla1*, Narasimha Reddy Yella1 and Kalyani Putty2
1Department of Veterinary Microbiology, 2Department of Veterinary Biotechnology, College of Veterinary Science, Rajendranagar, Hyderabad-500030, India
*Corresponding author
Abstract:

CPV-2 infection in dogs is recognized as one of the most common diseases especially in young puppies of less than a year age. Keeping in view of frequency of the occurrence of infection, sometimes even in vaccinated animals, the present study was taken up with the objective of standardizing the Real time PCR for easy and rapid diagnosis of the CPV-2 virus. In the present investigation a total of 100 faecal samples/rectal swabs were collected from dogs suffering with gastrointestinal disorders and showing the symptoms of canine parvovirus, like bloody diarrhoea, vomiting and pyrexia. The samples were tested initially with SYBR green dye based real time PCR with different combinations of the cycles and the results obtained were further confirmed by conventional PCR. We could able to standardize the real time PCR which was more helpful in screening of the faecal samples further in large numbers.


Keywords: Real time PCR, Canine parvovirus, Conventional PCR, SYBR green dye, Phenol chloroform method, DNA extraction
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How to cite this article:

Srinivas Manchikatla1, Narasimha Reddy Yella1 and Kalyani Putty. 2020. Standardization of SYBR Green Based Real Time PCR for Detection of Canine Parvovirus-2 (CPV-2).Int.J.Curr.Microbiol.App.Sci. 9(9): 3716-3722. doi: https://doi.org/10.20546/ijcmas.2020.909.458