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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 3540-3551
DOI: https://doi.org/10.20546/ijcmas.2020.909.439


Detection of Aflatoxins in Milk and Feed from Cases of Reproductive Tract Disorders in Cattle and Sheep
Bhupinder Kaur Khosa*, Tejinder Singh Rai, Narinder Singh Sharma
and Anil Kumar Arora
Department of Veterinary Microbiology, GADVASU, Ludhiana-141004, India
*Corresponding author
Abstract:

In this study, a total number of 135 samples were collected and out of them 80 samples were from reproductive tract, 25 feed samples and 30 milk samples from cattle and sheep. Samples collected from reproductive tract of cattle and sheep were subjected to bacteriological and fungal examination for isolation and identification. A total of 105 bacterial isolates have been found from 68 primary bacterial cultures out of which 50 isolates (47.61%) were found to be Gram-positive and 55 isolates (50.47%) were Gram-negative. Out of mixture of gram positive isolates, 31 isolates (62%) were confirmed to be Bacillus spp., 14 isolates (28%) Staphylococcus spp., 3 isolates (6%) Streptococcus spp. and 2 isolates (4%) were Arcanobacterium  pyogenes. Among all Gram-negative isolates, 3 isolates were found to positive for Brucella abortus, 30 for E. coli, 13 for Klebsiella pneumoniae, 7 for Proteus spp. and 2 for Pseudomonas aeruginosa. Amid abortion specific bacteria, 3 isolates have been confirmed to be positive for Brucella sps. by polymerase chain reaction using genus specific primers. A total of 34 fungal isolates were obtained from 68 fungal cultures, out of which a total of 8 fungal species from 6 genera have been identified. Major proportion of these isolates belong to Aspergillus spp. (41.17%), followed by Alternaria spp. (26.47%), Penicillium spp. (8%), Pithomyces spp. (8%) and Rhizopus spp. (8%) and Mucor spp. (5.8%). and are confirmed by molecular method using genus and species specific primers. Collected 25 feed and 30 milk samples were examined for aflatoxin contamination. A total of four feed samples were found to have aflatoxin contamination above the permissible limit i.e. 30ppb according to Indian standards. The level of aflatoxin contamination in feed samples was in between of 4 ppb and 52 ppb. All 30 collected milk samples were tested negative for aflatoxin contamination.


Keywords: Brucella, Aflatoxin, PCR, Milk, Feed
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How to cite this article:

Bhupinder Kaur Khosa, Tejinder Singh Rai, Narinder Singh Sharma and Anil Kumar Arora. 2020. Detection of Aflatoxins in Milk and Feed from Cases of Reproductive Tract Disorders in Cattle and Sheep.Int.J.Curr.Microbiol.App.Sci. 9(9): 3540-3551. doi: https://doi.org/10.20546/ijcmas.2020.909.439