National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study was conducted to evaluate the effect of tacrolimus 0.1% ointment on the tear fluid cytokines during treatment of keratoconjunctivitis sicca in dogs. Total 35 pug dogs were screened and graded as normal, early and late KCS cases on the basis of schirmer tear test. The concentration of the IFN-γ and immunohistochemistry (IHC) expression of MMP-9 were higher in KCS cases compared to normal cases. The effect of tacrolimus 0.1% ointment was studied on 16 KCS cases out of 35 which were divided into two groups A1 and A2 having 8 animals in each. Eye drops carboxy-methyl-cellulose and gentamicin was used in group A1 and ointment Tacrolimus 0.1% and drop gentamicin in group A2. The tear production was significantly (PË‚0.05) improved in A2 (Tacrolimus 0.1%) group whereas western blot expression of MMP-9 was more in group A1 post treatment. The concentration of the IL-8 and IFN-g was found to be decreased significantly (PË‚0.05) in A2 group post treatment. On the basis of results it was concluded that cytokines interferon- gamma (IFN-γ) and MMP-9 up-regulates in early and late stages of KCS and application of Tacrolimus (0.1%) ointment decreases the level of cytokines IL-8, IFN-γ and MMP-9 in KCS cases.
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