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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 3088-3094
DOI: https://doi.org/10.20546/ijcmas.2020.909.381


Prevalence and Characterization of Bacillus cereus in Raw Poultry Meat Sold at Retail Meat Outlets of Bikaner
Paramjeet Kaur*, Rakesh Rao, Sunil Meherchandani Rajani Joshi,
Manohar Sain and Pankaj Mangal
Department of Veterinary Public Health, College of Veterinary and Animal Science, RAJUVAS, Bikaner- 334001, Rajasthan, India
*Corresponding author
Abstract:

The present study was carried out to isolate Bacillus cereus from meat collected from Bikaner. In this study 50 raw poultry meat samples were collected from various retail meat outlets of Bikaner city. Out of 50 raw poultry meat samples, 14 (28%) samples were found positive for Bacillus cereus by culture and biochemical tests. The isolates showing positive reaction for the Catalase test, Voges Proskauer’s test, Arginine test, Nitrate reduction test, Citrate utilization test and sugar tests like Glucose, Sucrose, Arabinose and Trehalose while negative with ONPG test, Mannitol and Malonate test were confirmed as Bacillus cereus. Phenotypically identified B. cereus isolates were further analysed by polymerase chain reaction technique. The 14 isolates were subjected to PCR. Out of 14, 12 (85.71%) isolates possessed gyrB gene and were confirmed as B. cereus. Hence it was concluded that the occurrence of B. Cereus in foods especially in raw meat as a meat contaminant and causes food poisoning which is a serious public health concern and need to be addressed.


Keywords: Poultry, Bacillus cereus, Polymerase chain reaction, Food poisoning
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How to cite this article:

Paramjeet Kaur, Rakesh Rao, Sunil Meherchandani Rajani Joshi, Manohar Sain and Pankaj Mangal. 2020. Prevalence and Characterization of Bacillus cereus in Raw Poultry Meat Sold at Retail Meat Outlets of Bikaner.Int.J.Curr.Microbiol.App.Sci. 9(9): 3088-3094. doi: https://doi.org/10.20546/ijcmas.2020.909.381