National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Hospital indoor air and surfaces are good sources of bacteria, viruses and fungi which can lead to Hospital acquired infections (HAI) in susceptible hosts. Effective environmental disinfection and HVAC system maintenance are vital in preventing HAI. This study was aimed at estimating baseline data of bacterial and fungal bio burden during different seasons in a year and to strengthen infection control practices as there was an external construction work behind our hospital. A retrospective analysis of air surveillance data over a period of one year from July 2015 to June 2016 at a quaternary care hospital was done. Sampling was done with air petri sampler to check the quality of indoor and outdoor air once a month at 21 fixed locations. Nutrient agar plates (NA) and Sabouraud’s Chloramphenicol agar (SCA) plates were used. Bacteria and fungi suspended in air impinge on the agar surface. NA and SCA plates were incubated at 370C, 48 hours and 250C, 7 days respectively. Number of CFU/m3 calculated based on the air sampler manufacturer’s instructions. Average bacterial CFU/m3 in operation theatres and ICU was 29.65 and 93.85 respectively and that of fungi was 2.27 and 3.27 respectively. The maximum bacterial load was in summer and maximum fungal load was in south west monsoon. Aspergillus spp. was found throughout the year. Yeast and dematiaceous fungi like Curvularia spp., Alternaria spp. and Cladophialophora spp. were mostly found indoor during monsoons.
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