International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 9, Issue:9, September, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : /
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 2376-2389

Aero - surveillance of Various Units in a Quaternary Care Hospital Based on Seasonal Perspective – An Observational Study
Lokeshwari1, G. Balajee1 and T. Premalatha2*
1Department of Microbiology, 2Department of Biostatistics, Gleneagles Global Health City,No. 439, Cheran Nagar, Perumbakkam, Chennai, Tamil Nadu 600100
*Corresponding author

Hospital indoor air and surfaces are good sources of bacteria, viruses and fungi which can lead to Hospital acquired infections (HAI) in susceptible hosts. Effective environmental disinfection and HVAC system maintenance are vital in preventing HAI. This study was aimed at estimating baseline data of bacterial and fungal bio burden during different seasons in a year and to strengthen infection control practices as there was an external construction work behind our hospital. A retrospective analysis of air surveillance data over a period of one year from July 2015 to June 2016 at a quaternary care hospital was done. Sampling was done with air petri sampler to check the quality of indoor and outdoor air once a month at 21 fixed locations. Nutrient agar plates (NA) and Sabouraud’s Chloramphenicol agar (SCA) plates were used. Bacteria and fungi suspended in air impinge on the agar surface. NA and SCA plates were incubated at 370C, 48 hours and 250C, 7 days respectively. Number of CFU/m3 calculated based on the air sampler manufacturer’s instructions. Average bacterial CFU/m3 in operation theatres and ICU was 29.65 and 93.85 respectively and that of fungi was 2.27 and 3.27 respectively. The maximum bacterial load was in summer and maximum fungal load was in south west monsoon. Aspergillus spp. was found throughout the year. Yeast and dematiaceous fungi like Curvularia spp., Alternaria spp. and Cladophialophora spp. were mostly found indoor during monsoons.

Keywords: Hospital air sampling, Seasonal bio-burden, Surveillance, Microbial count, Hospital air quality, Aspergillus

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How to cite this article:

Lokeshwari, G., G. Balajee and Premalatha, T. 2020. Aero - surveillance of Various Units in a Quaternary Care Hospital Based on Seasonal Perspective – An Observational Study.Int.J.Curr.Microbiol.App.Sci. 9(9): 2376-2389. doi:
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