National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
E. coli are normal inhabitants of the gastrointestinal tract of animals, birds and humans of which only some strains have become highly adapted to cause diarrhoea and a range of extra-intestinal diseases. In the present study, a total of 320 raw poultry meat samples were collected from retail poultry meat market of Bhubaneswar, Odisha, India. A total of 224 E. coli isolates were obtained. The triturated samples were first inoculated on MLA and then on EMB agar, the colonies with greenish metallic sheen were considered as E. coli which was further confirmed by biochemical tests. The overall prevalence of E. coli observed during the present study was 70 per cent in the samples. In vitro antibiotic resistance patterns against 18 antibiotics revealed highest resistance to Oxytetracycline and highest sensitivity was exhibited to Tobramycin by all types of isolates. All the 224 E. coli isolates were subjected to biofilm production by Microtiter Plate Method and Congo Red Agar method. 68.75% of E. coli isolates were found to be positive for biofilm formation by both MTP method and CRA method. By MTP method 12.5%, 79.17% and 8.33% isolates are high, moderate and weakly positive respectively. By CRA method, 20.83%, 50% and 29.17% isolates are high, moderate and weakly positive respectively.
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