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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 1608-1614
DOI: https://doi.org/10.20546/ijcmas.2020.909.200


Isolation and Molecular Characterization of Mycoplasma Isolates from Pneumonic Sheep and Goats in Andhra Pradesh
Monika Yadav1, S. Vijaya Lakshmi2*, N. Vinod Kumar3 and A. Jagadeesh Babu4
1Veterinary Assistant Surgeon, Nagalapuram Chittoor (dt), AP, India
2LRS, Palamaneru, SVVU, India
3Department of Microbiology, CVSc, Tirupati, India
4Department of Veterinary Public health & Epidemiology, CVSc, Tirupati, India
*Corresponding author
Abstract:

In the present study, attempts for the isolation of Mycoplasma organisms were carried out in PPLO broth and PPLO agar medium. Out of 253 sheep and 112 goat nasal swab samples inoculated in PPLO broth, 27 sheep and 14 goat samples showed colour change in broth. Upon sub-culturing of 27 sheep and 14 goat broth nasal swab samples on PPLO agar, 17 sheep and 9 goat samples produced colonies. Positive samples produced yellow color change to broth medium with mild turbidity after 48 hrs of incubation at 37°C in CO2 incubator maintaining 5% CO2. After 7th day of incubation on PPLO agar, the agar plates showed fried egg colonies and granular colonies when examined under 10X magnification of microscope. Further Mycoplasma isolation was confirmed by PCR targeting 16S rRNA gene of genus Mycoplasma and Mycoplasma ovipneumoniae.


Keywords: Pneumonic sheep and goats, Mycoplasma
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How to cite this article:

Monika Yadav, B., S. Vijaya Lakshmi, N. Vinod Kumar and Jagadeesh Babu, A. 2020. Isolation and Molecular Characterization of Mycoplasma Isolates from Pneumonic Sheep and Goats in Andhra Pradesh.Int.J.Curr.Microbiol.App.Sci. 9(9): 1608-1614. doi: https://doi.org/10.20546/ijcmas.2020.909.200