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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(9): 1167-1179
DOI: https://doi.org/10.20546/ijcmas.2020.909.145


Gamma Irradiation and NaCl Treatment on the Embryonic Callus of Rice (Oryza sativa L.) Cultivar Kuliyadichan and CO 43 for Salt Tolerance
Venkateshan1, S. Merina Prem Kumari1*, M. Arumugam Pillai1,Saravanan1 and A. Kavitha Pushpam2
1Department of Plant Breeding and Genetics, 2Department of Soil Science and Agricultural Chemistry, Agricultural College and Research Institute, Killikulam,
Vallanad-628252, Tamil Nadu, India
*Corresponding author
Abstract:

The present study investigated the effect of gamma irradiation and NaCl treatment on the embryonic calli of indica rice variety Kuliyadichan and CO43. Embryonic calli obtained from both the varieties were exposed to gamma irradiation (0, 20, 30, 40 and 50 Gy) and lethal dose LD50 based on calli survival and regeneration capacity was determined. The LD50 based on calli survival was deduced as 34.31 Gy and 31.01 Gy for Kuliyadichan and CO43 respectively. Similarly the LD50 based on regeneration capacity was deduced as27.09 Gy and 24.01 Gy for variety Kuliyadichan and CO43. Furthermore, embryonic calli from both varieties were exposed to four different NaCl concentrations with electrical conductivity of 4, 8, 10 and 12 dS/m and the lethal concentration LC50 based on calli survival and regeneration capacity was determined. The lethal concentration LC50 based on calli survival was fixed as 11.01 dS/m for Kuliyadichan and 10.24 dS/m for CO43. Similarly, the LC50 based on regeneration was fixed as 10.6 dS/m for variety Kuliyadichan and 9.19 dS/m for variety CO43. Based on overall consideration, the variety CO43 showed more sensitivity to gamma irradiation and NaCl stress than the variety Kuliyadichan.


Keywords: Rice, Embryonic calli, Gamma irradiation, NaCl stress, LD50 and LC50
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How to cite this article:

Venkateshan, R., S. Merina Prem Kumari, M. Arumugam Pillai, S. Saravanan and Kavitha Pushpam, A. 2020. Gamma Irradiation and NaCl Treatment on the Embryonic Callus of Rice (Oryza sativa L.) Cultivar Kuliyadichan and CO 43 for Salt Tolerance.Int.J.Curr.Microbiol.App.Sci. 9(9): 1167-1179. doi: https://doi.org/10.20546/ijcmas.2020.909.145