National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study investigated the effect of gamma irradiation and NaCl treatment on the embryonic calli of indica rice variety Kuliyadichan and CO43. Embryonic calli obtained from both the varieties were exposed to gamma irradiation (0, 20, 30, 40 and 50 Gy) and lethal dose LD50 based on calli survival and regeneration capacity was determined. The LD50 based on calli survival was deduced as 34.31 Gy and 31.01 Gy for Kuliyadichan and CO43 respectively. Similarly the LD50 based on regeneration capacity was deduced as27.09 Gy and 24.01 Gy for variety Kuliyadichan and CO43. Furthermore, embryonic calli from both varieties were exposed to four different NaCl concentrations with electrical conductivity of 4, 8, 10 and 12 dS/m and the lethal concentration LC50 based on calli survival and regeneration capacity was determined. The lethal concentration LC50 based on calli survival was fixed as 11.01 dS/m for Kuliyadichan and 10.24 dS/m for CO43. Similarly, the LC50 based on regeneration was fixed as 10.6 dS/m for variety Kuliyadichan and 9.19 dS/m for variety CO43. Based on overall consideration, the variety CO43 showed more sensitivity to gamma irradiation and NaCl stress than the variety Kuliyadichan.
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